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23-021M UBE1 Protein, active, 250 µg

Active, full length, recombinant human UBE1. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E1 enzyme in ubiquitination assays.

UBE1 Protein, active, 250 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Key Applications: Enzyme Assays
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23-021M
250 µg  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Key ApplicationsSpeciesCascade Component
      Enzyme AssaysHuman E1
      Description
      Catalogue Number23-021M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionUBE1 Protein, active, 250 µg
      OverviewN-terminal 6His-tagged, recombinant human UBE1 full length, expressed by baculovirus in Sf21 insect cells.
      Alternate Names
      • Ubiquitin activating enzyme E1
      • Ubiquitin-like modifier-activating enzyme 1
      • UBA1
      • A1S9 Protein
      Background InformationUBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It is responsible for the primary ubiquitin-protein isopeptide bond formation and is a critical component for the initiation of in vitro conjugation reactions. UBE1 activates ubiquitin by the ATP dependent adenylation of its C-terminal glycine carboxyl group. This ubiquitin residue is then linked to the sulphydryl side chain moiety of a cysteine residue within UBE1 by forming a high energy thiol ester bond, liberating free AMP. There are two active sites within the UBE1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, full length, recombinant human UBE1. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E1 enzyme in ubiquitination assays.
      Key Applications
      • Enzyme Assays
      Biological Information
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Cascade ComponentE1
      Entrez Gene Number
      Purification MethodPurified using immobilized metal affinity chromatography.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      23-021M 04053252632945

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      Categorías

      Life Science Research > Proteins and Enzymes > Ubiquitin E1 Activating Enzyme
      Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitin E1 Activating Enzyme