Millipore Sigma Vibrant Logo

560000 SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem

Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
View Products on Sigmaaldrich.com
560000
Ver precios y disponibilidad

Descripción

Replacement Information

Tabla espec. clave

CAS #
3483-12-3

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
560000-10ML
Comprobando disponibilidad...
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      		 Frasco de vidrio 10 ml
      Recuperando precio...
      No pudo obtenerse el precio
      La cantidad mínima tiene que ser múltiplo de
      Maximum Quantity is
      Al finalizar el pedido Más información
      Ahorró ()
       
      —
      Solicitar precio
      		 —
      560000-1SET
      Comprobando disponibilidad...
      —
      Disponibilidad a confirmar Disponibilidad a confirmar
      Ingrese cantidad 
      Suspendido
      Cantidades limitadas disponibles
      Debe confirmarse disponibilidad
        El resto: se avisará
          El resto: se avisará
          Se avisará
          Póngase en contacto con el Servicio de Atención al Cliente
          Contact Customer Service

          		 Frasco de vidrio 1 set
          Recuperando precio...
          No pudo obtenerse el precio
          La cantidad mínima tiene que ser múltiplo de
          Maximum Quantity is
          Al finalizar el pedido Más información
          Ahorró ()
           
          —
          Solicitar precio
          		 —
          Description
          OverviewConcentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.
          Note: 1 set = 20 x 10 ml.
          Catalogue Number560000
          Brand Family Calbiochem®
          References
          ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
          Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
          Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
          Cleland, W.W. 1964. Biochemistry 3, 480.
          May, J.R. 1953 (September). Lancet p. 535.
          Product Information
          CAS number3483-12-3
          FormLiquid
          Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSEK1610000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage +15°C to +30°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Número de referencia GTIN
          560000-10ML 04055977268195
          560000-1SET 07790788051556

          Documentation

          Licencias necesarias

          Título
          PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

          SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem Certificados de análisis

          CargoNúmero de lote
          560000

          Referencias bibliográficas

          Visión general referencias
          Hirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
          Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
          Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
          Cleland, W.W. 1964. Biochemistry 3, 480.
          May, J.R. 1953 (September). Lancet p. 535.

          Citas

          Título
        • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.
          		•
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-April-2008 RFH
          DescriptionA concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.
          FormLiquid
          Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
          Recommended reaction conditionsA. To prepare sputum samples: 1. In a screw-cap centrifuge tube, overlay the sputum sample with an equal volume of the diluted SPUTOLYSIN® Reagent. 2. Suspend the sputum by mixing on a vortex mixer for 30 s. 3. Allow the suspended mixture to stand at room temperature for 15 min. 4. Centrifuge the mixture for 5 min at 1500 rpm. Discard the supernatant. B. To prepare cultures for acid-fast bacilli: 1. Suspend the pellet from Step A(4) in 5 to 10 ml of 1% sodium hydroxide by mixing on a vortex mixer for 1 min. 2. Centrifuge the mixture for 15 min at 3000 rpm. Discard the supernatant. 3. Rinse the pellet twice in 10 ml of sterile normal saline to remove the residual sodium hydroxide. 4. Resuspend the pellet in 0.5 ml of the diluted SPUTOLYSIN® Reagent.
          CAS number3483-12-3
          RTECSEK1610000
          SolubilityDilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.
          Storage Protect from light
          +15°C to +30°C
          Do Not Freeze Ok to freeze
          Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
          Toxicity Harmful
          ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
          Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
          Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
          Cleland, W.W. 1964. Biochemistry 3, 480.
          May, J.R. 1953 (September). Lancet p. 535.
          Citation
        • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.
          		•