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HTS059RTA Ready-to-Assay™ V 1A Vasopressin Receptor Frozen Cells

Human V1A GPCR frozen cells for Calcium Flux FLIPR Assays.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Host Cell: Chem-1 Entrez Gene Number: NM_005242
HTS059RTA
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      Descripción

      Replacement Information

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      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Tabla espec. clave

      SpeciesHost CellsProtein Target
      Human Chem-1V1A
      Description
      Catalogue NumberHTS059RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ V 1A Vasopressin Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells vwith high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      Arginine vasopressin (AVP) is a 9 amino acid peptide that functions as an antidiuretic, vasoconstrictor and neurotransmitter. The three vasopressin receptors, V1A, V1B and V2, are GPCRs; V1A and V1B couple to Gq and calcium release, whereas V2 couples to Gs. V1 receptors regulate fluid homeostasis by stimulating contraction of glomerular mesangial cells and renal vascular smooth muscle cells (Birnbaumer, 2000). Millipore’s cloned human V1A -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant V1A expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at V1A Receptor.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ100
      Applications
      ApplicationHuman V1A GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsChem-1
      Protein TargetV1A
      Target Sub-FamilyVasopressin
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      HTS059RTA 04053252481475

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      Categorías

      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells