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HTS020RTA Ready-to-Assay™ CB2 Cannabinoid Receptor Frozen Cells

Human CB2 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-4 Entrez Gene Number: X74328
HTS020RTA
1 kit  
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      Descripción

      Replacement Information

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      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Tabla espec. clave

      SpeciesHost CellsProtein Target
      Human Chem-4CB2
      Description
      Catalogue NumberHTS020RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ CB2 Cannabinoid Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      Cannabinoid compounds include exogenous drugs such as 9-THC, the main psychoactive component of the plant Cannabis sativa, and endogenous mediators, such as anandamide, that belong to eicosanoid family. The biological effects of cannabinoids are mediated by a family of two Gi-coupled 7-transmembrane receptors, CB1 and CB2. The CB1 receptor is found primarily in brain and mediates the psychoactive effects of cannabinoid ligands. The CB2 receptor is expressed mainly in immune cells, including mast cells and CD40-activated B cells, where it mediates proliferation and inhibition of migration (Howlett et al., 2002). Activation of CB2 inhibits the development of liver fibrosis (Julien et al., 2005). In bone, CB2 is expressed in both osteoblasts and osteoclasts, and functions to prevent bone loss (Ofek et al., 2006). In addition, activation of CB2 has an antinociceptive effect in animal models of neuropathic, inflammatory, and acute pain; this effect is mediated by release of endogenous opioids in the periphery (Ibrahim et al., 2005). Millipore’s cloned human CB2-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant CB2 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at CB2.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ300
      Applications
      ApplicationHuman CB2 GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsChem-4
      Protein TargetCB2
      Target Sub-FamilyCannabinoid
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      HTS020RTA 04053252629990

      Documentation

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      Categorías

      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells