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540221 Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

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540221
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540221-1MG
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      Description
      OverviewRecombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. This reaction is reversible. The human enzyme is a target for therapeutic immune response intervention.
      Catalogue Number540221
      Brand Family Calbiochem®
      SynonymsPNP
      References
      ReferencesFilgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun. 309, 917.
      Silva, R.G., et al. 2003. Protein Expr. Purif. 27, 158.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P<sub>i</sub> into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.
      FormLiquid
      FormulationIn 50 mM Tris-HCl, pH 7.6.
      Quality LevelMQ100
      Biological Information
      PuritySingle band by SDS-PAGE
      Specific Activity≥25 units/mg protein
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Global Trade Item Number
      Número de referencia GTIN
      540221-1MG 04055977268751