Millipore Sigma Vibrant Logo

539125 Protease Assay Kit

View Products on Sigmaaldrich.com
539125
Ver precios y disponibilidad

Descripción

Replacement Information

Tabla espec. clave

Detection Methods
Colorimetric

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
539125-1KIT
Comprobando disponibilidad...
Disponibilidad a confirmar
Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      Frasco de vidrio 1 kit
      Recuperando precio...
      No pudo obtenerse el precio
      La cantidad mínima tiene que ser múltiplo de
      Maximum Quantity is
      Al finalizar el pedido Más información
      Ahorró ()
       
      Solicitar precio
      Description
      OverviewA kit suitable for quantitative array of a wide variety of proteases in biological samples, utilizing FTC-casein as a substrate. Enzyme activities that have been detected with this assay kit include: chymotrypsin, elastase, plasminogen, PRONASE® Protease, subtilisin, thermolysin, and trypsin.
      Catalogue Number539125
      Brand Family Calbiochem®
      Materials Required but Not Delivered Trichloroacetic acid (TCA), 5%
      Spectrophotometer that can accurately measure the absorbance at 492 nm
      Pipetting devices for accurate delivery of volumes required for the assay
      Cuvettes with optical properties suitable for use at 492 nm
      A constant-temperature water bath set at 37°C
      Microfuge for generating approximately 12,000 x g
      References
      ReferencesTwining, S.S. 1984. Anal. Biochem. 143, 30.
      Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.
      Product Information
      Detection methodColorimetric
      Form200 Tests
      FormatCuvette or 96-well plate
      Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
      Quality LevelMQ100
      Applications
      Biological Information
      Assay time3 h
      Sample TypeBiological samples
      Physicochemical Information
      Sensitivity10 ng/ml
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Storage Conditions FTC-casein should be stored at -20°C and is stable for one year from the date of purchase. FTC-casein stored at 4°C is stable for 2-3 days. Several freeze-thaw cycles will lead to a minor increase in the background fluorescence, so following initial thawing, we suggest that this reagent be divided into 5 aliquots. FTC-casein is light sensitive and should be protected from light.
      Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C.
      Incubation buffer and assay buffer may be stored at 4°C or -20°C.
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      539125-1KIT 04055977195347

      Documentation

      Protease Assay Kit Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Protease Assay Kit Certificados de análisis

      CargoNúmero de lote
      539125

      Referencias bibliográficas

      Visión general referencias
      Twining, S.S. 1984. Anal. Biochem. 143, 30.
      Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.

      Folleto

      Cargo
      Kit SourceBook - 2nd Edition EURO
      Kits SourceBook - 2nd Edition GBP

      Protocolos de usuario

      Cargo
      User Protocol