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Paneles personalizados y kits premezclados
Nuestra amplia cartera de productos consta de paneles multiplex que le permiten elegir, dentro del panel, los analitos que mejor se ajustan a sus requisitos. En una pestaña distinta puede elegir el formato de citocina premezclada o un kit single plex.
Kits de señalización celular y MAPmates™
Elija los kits preparados para poder explorar las vías o los procesos enteros. O diseñe sus propios kits eligiendo single plex MAPmates™ según las directrices proporcionadas.
No deben combinarse los siguientes MAPmates™: -MAPmates™ que requieren un tampón de ensayo diferente. -Pares MAPmate™ fosfoespecíficos y totales, por ejemplo, GSK3β y GSK3β (Ser 9). -MAPmates™ con panTyr y específicos de sitio; por ejemplo, receptor del fosfo-EGF y fosfo-STAT1 (Tyr701). -Más de 1 fosfo-MAPmate™ para una sola diana (Akt, STAT3). -La GAPDH y la β-tubulina no pueden combinarse con kits o MAPmates™ que contengan panTyr.
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96-Well Plate
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Añadir más reactivos (Se necesita tampón y un kit de detección para usar con MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Opción para ahorrar espacio Los clientes que adquieran múltiples kits pueden optar por ahorrar espacio de almacenamiento retirando el embalaje del kit y recibiendo los componentes de sus ensayos multiplex en bolsas de plástico para un almacenamiento más compacto.
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17-499
Sigma-AldrichPhosphorylation Explorer Pack Sample Kit
This Antibody pack contains Anti-phosphotyrosine Antibody, Anti-Phosphoserine Antibody, validated for use in WB, IF, Flow, IHC, ELISA, ICC.
More>>This Antibody pack contains Anti-phosphotyrosine Antibody, Anti-Phosphoserine Antibody, validated for use in WB, IF, Flow, IHC, ELISA, ICC. Less<<
Phosphorylation Explorer Pack Sample Kit: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
4G10® Platinum The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
4A4 The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).
This Antibody pack contains Anti-phosphotyrosine Antibody, Anti-Phosphoserine Antibody, validated for use in WB, IF, Flow, IHC, ELISA, ICC.
Key Applications
Western Blotting
Immunofluorescence
Flow Cytometry
Immunohistochemistry
ELISA
Immunocytochemistry
Biological Information
Clone
4G10® Platinum and 4A4
Host
Mouse
Modifications
Phosphorylation
Molecular Weight
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
See individual components for storage/handling conditions.
Packaging Information
Material Size
1 kit
Global Trade Item Number
Número de referencia
GTIN
17-499
04053252009914
Documentation
Phosphorylation Explorer Pack Sample Kit Ficha datos de seguridad (MSDS)
Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more. Más información >>
Phosphoserine/Threonine Antibodies & Kits
Approximately 400 of the 518 kinases in the human genome are serine/threonine specific protein kinases. Millipore offers antibodies for your phospho-Ser/Thr research needs, such as those below. Más información >>
Phosphoserine Antibodies
Millipore’s phosphoserine antibodies, including the popular 4A4 clone, demonstrate sensitivity and detect multiple serine phosphorylations on numerous substrates. See below for pSer antibodies. Más información >>
Phosphotyrosine Antibodies
Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies. Más información >>