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539482 PKA, His•Tag®, Active, Human, Recombinant, E. coli

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Protein Kinase A, cAMP Dependent Protein Kinase

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      Descripción

      Description
      Overview

      This product has been discontinued.



      Recombinant, human, catalytic subunit of PKA fused to at the N-terminus to a His•Tag® sequence and expressed in E.coli. PKA regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for labeling PKA substrates.
      Catalogue Number539482
      Brand Family Calbiochem®
      SynonymsProtein Kinase A, cAMP Dependent Protein Kinase
      Application Data
      Increasing amounts of PKA were subjected to SDS-PAGE and purity analyzed by Coomassie Blue staining.

      Increasing amounts of PKA were analyzed for activity as outlined in the protocol above.
      References
      ReferencesSkalhegg, B.S. and Tasken K., et al. 2000. Front. Biosci. 5, D678.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to PKA peptide (GRTGRRNSI) per min at 30°C.
      FormLiquid
      FormulationIn 150 mM KCl, 30 mM K₂HPO₄, 1 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.4.
      Quality LevelMQ100
      Biological Information
      Purity≥95% by SDS PAGE
      Specific Activity≥ 500,000 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Número de referencia GTIN
      539482 0