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539481 PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: cAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit

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539481
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539481-100KU
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      Description
      OverviewRecombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
      Catalogue Number539481
      Brand Family Calbiochem®
      SynonymscAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
      References
      ReferencesGrant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
      Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
      Adams, J.A., et al. 1995. Biochemistry 34, 2447.
      Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
      Brindle, P., et al. 1993. Nature 364, 821.
      Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
      Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
      Wang, L-Y., et al. 1993. Science 259, 1173.
      Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
      Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
      Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.
      Product Information
      Activity≥2.5 x 10⁶ U/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; <a href ="/Products/ProductDisplay.asp→catNO=05-23-4900">Cat. No. 05-23-4900</a>) per min at 30°C, pH 7.5.
      EC number2.7.1.37
      FormLiquid
      FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
      Quality LevelMQ100
      Physicochemical Information
      ContaminantsPhosphatases, proteases: none detected
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Número de referencia GTIN
      539481-100KU 04055977269178