Millipore Sigma Vibrant Logo

14-604M-D PI3 kinase (p110δ/p85α)

Complex of N-terminal GST recombinant full-length human p110δ, and recombinant full length, human p85α, coexpressed in a Baculovirus infected Sf9 cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

PI3 kinase (p110δ/p85α): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
14-604M-D
250 μg  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information
      Description
      Catalogue Number14-604M-D
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPI3 kinase (p110δ/p85α)
      OverviewComplex of N-terminal GST recombinant full-length human p110δ, and recombinant full length, human p85α, coexpressed in a Baculovirus infected Sf9 cell expression system
      References
      Product Information
      Presentation25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.03% Tween-20, 3 mM DTT, and 50% glycerol
      Quality LevelMQ100
      Applications
      ApplicationComplex of N-terminal GST recombinant full-length human p110δ, and recombinant full length, human p85α, coexpressed in a Baculovirus infected Sf9 cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
      Key Applications
      • Kinase Assay
      Biological Information
      Purity≥75%
      SourceSf9 insect cells
      Specific Activity500 pmol/min/µg.
      Entrez Gene Number
      Entrez Gene SummaryPhosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. See MIM 602838. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha (MIM 171834), p110-beta (MIM 602925), and p110-gamma (MIM 601232). p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins (see MIM 171833) and with GTP-bound Ras.[supplied by OMIM]
      Gene Symbol
      • PIK3CD
      • p110d
      • PI3K
      • p110D
      • p110delta
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummarySIZE: 1044 amino acids; 119479 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Interacts with ERAS (By similarity).
      TISSUE SPECIFICITY: Expressed predominantly in leukocytes.
      PTM: Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
      SIMILARITY: SwissProt: O00329 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
      Molecular Weight146 kDa (p110δ), 84 kDa (p85α)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Kinase assay: A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 100 uM ATP, 200 μM PI:PS substrate and p110δ/p85α for 17 minutes at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for > 6 month at –80°C from date of receipt.
      Packaging Information
      Material Size250 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      14-604M-D 04055977276633