Millipore Sigma Vibrant Logo

521270 PDK1, His•Tag®, Human, Recombinant, S. frugiperda

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: 3-Phosphoinositide-Dependent Protein Kinase-1

View Products on Sigmaaldrich.com
521270
  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information
      Description
      OverviewFull length, recombinant, human PDK1 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
      Catalogue Number521270
      Brand Family Calbiochem®
      Synonyms3-Phosphoinositide-Dependent Protein Kinase-1
      References
      ReferencesScott, M.T., et al. 2002. EMBO J. 21, 6771.
      Biondi, R.M., et al. 2000. EMBO J. 19, 979.
      Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
      EC number2.7.10
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥75 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      521270 0

      Documentation

      PDK1, His•Tag®, Human, Recombinant, S. frugiperda Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      PDK1, His•Tag®, Human, Recombinant, S. frugiperda Certificados de análisis

      CargoNúmero de lote
      521270

      Referencias bibliográficas

      Visión general referencias
      Scott, M.T., et al. 2002. EMBO J. 21, 6771.
      Biondi, R.M., et al. 2000. EMBO J. 19, 979.
      Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.

      Folleto

      Cargo
      Biologics 31.1
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-August-2014 JSW
      Synonyms3-Phosphoinositide-Dependent Protein Kinase-1
      DescriptionFull length, recombinant, human PDK1 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      EC number2.7.10
      Purity≥90% by SDS-PAGE
      Specific activity≥75 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesScott, M.T., et al. 2002. EMBO J. 21, 6771.
      Biondi, R.M., et al. 2000. EMBO J. 19, 979.
      Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.