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14-452-D PDK1

Recombinant human PDK1, full length, with N-terminal GST tag, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

PDK1: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
14-452-D
10 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue Number14-452-D
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPDK1
      OverviewRecombinant human PDK1, full length, with N-terminal GST tag
      References
      Product Information
      Presentation40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
      Quality LevelMQ300
      Applications
      ApplicationRecombinant human PDK1, full length, with N-terminal GST tag, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
      Key Applications
      • Kinase Assay
      Biological Information
      Concentration0.5 mg/ml
      Purity≥80%
      SourceSf9 insect cells
      Specific Activity5.02 pmol/min/µg
      Entrez Gene Number
      Gene Symbol
      • PDPK1
      • MGC20087
      • PDK1
      • PkB-like
      • PRO0461
      • MGC35290
      • hPDK1
      Protein TargetPDK1
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
      SIZE: 556 amino acids; 63152 Da
      SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
      TISSUE SPECIFICITY: Appears to be expressed ubiquitously.
      PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
      SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight89 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Kinase assay: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 EM ATP, 0.01% Brij-35 and 2 EM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for > 6 month at –80°C from date of receipt.
      Packaging Information
      Material Size10 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      14-452-D 04055977276565