Millipore Sigma Vibrant Logo

509290 OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

G418 is an aminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: NMD Inhibitor I

509290
  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information

      Tabla espec. clave

      CAS #Empirical Formula
      108321-42-2C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Description
      OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
      Catalogue Number509290
      Brand Family Calbiochem®
      SynonymsNMD Inhibitor I
      References
      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
      Product Information
      CAS number108321-42-2
      FormWhite powder
      Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Quality LevelMQ200
      Applications
      Biological Information
      Biological activityAssay: ≥730 µg/mg
      Purity≥90% by HPLC
      Physicochemical Information
      ContaminantsHeavy metals: ≤100 ppm
      UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSCB9378500
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      509290 0

      Documentation

      OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificados de análisis

      CargoNúmero de lote
      509290

      Referencias bibliográficas

      Visión general referencias
      Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-March-2020 JSW
      SynonymsNMD Inhibitor I
      DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
      FormWhite powder
      Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
      CAS number108321-42-2
      RTECSCB9378500
      Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
      Purity≥90% by HPLC
      ContaminantsHeavy metals: ≤100 ppm
      UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
      Biological activityAssay: ≥730 µg/mg
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
      Toxicity Standard Handling
      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.