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SCC456 OSUMMER.12 Mouse NRAS-Mutant Melanoma Cell Line

The OSUMMER cell lines are responsive to immunotherapy treatments. These NRAS-Mutant cell lines can be used to discover and address potential immunotherapies in NRAS-mutant human melanomas which make up an estimated 15-25% of human melanomas. These cell lines also enable in vivo testing of immunotherapies with a mouse model. OSUMMER.12 carries the specific NRAS mutation Q61R and can form tumors in vivo without Matrigel injection.

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SCC456
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      Descripción

      Replacement Information
      Description
      Catalogue NumberSCC456
      DescriptionOSUMMER.12 Mouse NRAS-Mutant Melanoma Cell Line
      Background InformationMelanoma skin cancer develops from melanocytes, a cell type found in the skin that is involved in melanin production and that influences pigmentation. While skin cancers are the most common type of cancers, melanoma is less common, accounting for only about 1% of skin cancer cases. Melanoma is nonetheless a significant form of skin cancer due to its capability to metastasize to other tissues if not detected in early stages. Early-stage melanoma can be cured by resection, but fewer treatment options are available for patients with metastatic disease. Development of resistance to targeted and immunotherapies, as well as toxicity are major problems. Along with sun exposure, sporadic genetic mutations have been associated with melanomas. Immunotherapy treatments targeting these genes or pathways have been proven to have significant benefits in melanoma patients by improving general response and survival.
      These NRAS-Mutant cell lines can be used to discover and address potential immunotherapies in NRAS-mutant human melanomas which make up an estimated 15-25% of human melanomas. These cell lines also enable in vivo testing of immunotherapies with a mouse model.
      OSUMMER.12 NRAS mouse melanoma cell line was derived from a female TN mouse exposed to UVA and UVB radiation. OSUMMER.12 carries the specific NRAS mutation Q61R. WES revealed high SNV (Single Nucleotide Variants) and low CNA (Copy Number Alterations) burden, as well as the presence of UV signature mutations.

      Source
      OSUMMER.12 was derived from melanoma induced in TN (Tyr::CreER) mice exposed to UVA or UVB radiation on postnatal day 3.

      References
      1. Nat Rev Dis Primers 2015, 1: 15003.
      2. Lancet 2018, 392(10151): 971-984.
      3. Life Sci Alliance 2021, 4(9): e202101135.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationThe OSUMMER cell lines are responsive to immunotherapy treatments. These NRAS-Mutant cell lines can be used to discover and address potential immunotherapies in NRAS-mutant human melanomas which make up an estimated 15-25% of human melanomas. These cell lines also enable in vivo testing of immunotherapies with a mouse model. OSUMMER.12 carries the specific NRAS mutation Q61R and can form tumors in vivo without Matrigel injection.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach vial contains ≥1X10⁶ viable cells.

      Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.

      Cells are verified to be of mouse origin and negative for interspecies contamination from rat, human, chinese hamster, Golden Syrian hamster, and nonhuman Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services

      Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsOSUMMER.12 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      SCC456 04065268032657