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14-545M NEK2 Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, full-length, human NEK2, for use in Kinase Assays.

NEK2 Protein, active, 250 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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14-545M
250 µg  Also available in 10 μg size (cat#14-545) and in bulk (cat# 14-545-K).
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      Descripción

      Description
      Catalogue Number14-545M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionNEK2 Protein, active, 250 µg
      OverviewN-terminal His6-tagged, recombinant, full-length, human NEK2
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive, N-terminal His6-tagged, recombinant, full-length, human NEK2, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • NEK2
      • NLK1
      • NLK
      • NEK2A
      • HsPK21
      Protein TargetNEK2
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyMisc
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P51955 # Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
      COFACTOR: Magnesium.
      SIZE: 445 amino acids; 51763 Da
      SUBUNIT: Interacts with TERF1. Isoform 1 and isoform 2 form homo- and heterodimers.
      SUBCELLULAR LOCATION: Nucleus. & Isoform 1: Nucleus, nucleolus. Note=Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end. & Isoform 2: Cytoplasm. Note=Predominantly cytoplasmic.
      TISSUE SPECIFICITY: Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
      DEVELOPMENTAL STAGE: Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Expression of both isoform 1 and isoform 2 is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase, whereas isoform 2 remains present.
      SIMILARITY: SwissProt: P51955 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. NIMA subfamily. & Contains 1 protein kinase domain.
      Molecular Weight52.8kDa
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of MBP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-545) and in bulk (cat# 14-545-K).
      Global Trade Item Number
      Número de referencia GTIN
      14-545M 04053252368059