Millipore Sigma Vibrant Logo

AAMT002 Mitochondrial Complex II Activity Assay Kit

Mitochondrial Complex II Activity Assay Kit: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
View Products on Sigmaaldrich.com
AAMT002
  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Product Description

      Complex II, also known as succinate-coenzyme Q reductase (SDH, EC 1.3.5.1), is one of the five complexes involved in oxidative phosphorylation in the inner mitochondrial membrane and also a member of the tricarboxylic acid cycle (TCA). It catalyzes electron transfer from succinate to the electron carrier, ubiquinone, but unlike the other four complexes it is not a proton pump. The product ubiquinol is utilized by complex III in the respiratory chain and the product fumarate is necessary to maintain the TCA cycle.



      Succinate + ubiquinone (Q) → Fumarate + ubiquinol (QH2)

      The Mitochondrial Complex II Activity Assay is designed for determining the Complex II activity in a sample. Each of the 96 wells in the kit has been coated with an anti-Complex II monoclonal antibody (mAb) which purifies the enzyme from a complex sample such as mitochondria, tissue homogenate or cell lysate. After this in-well purification the production of ubiquinol by the enzyme is coupled to the reduction of the dye DCPIP (2,6-diclorophenolindophenol) and a decreases in its absorbance at 600 nm which in turn recycles the substrate ubiquinone, as shown below.

      ubiquinol (QH2) + DCPIP (blue) → ubiquinone (Q) + DCPIPH2 (colorless)


      Panel Components

      15 ml 	20X Buffer, (72581-15ML)
25 ml	Complex II Activity Buffer, (72582-25ML)
2 x 1 ml	10X Detergent, (72583-1ML)
500 µl	50X Succinate Solution, (72584-500UL)
250 µl	100X DCPIP, (72585-250UL)
60 µl	Ubiquinone 2, (72586-60UL)
5 ml	10X Blocking Solution, (72587-5ML)
6 ml	Phospholipids, (72588-6ML)
1 ea	Microplate, (72589-1EA)

      * This kit contains sufficient materials for 96 measurements (one 96-well microplate).



      Storage: Store the 20X Buffer, Complex II Activity Buffer, 10X Detergent, 10X Blocking Solution, and Microplate at 4°C. Store the 50X Succinate Solution, 100X DCPIP,
      Ubiquinone 2, and Phospholipids at –80°C The Mitochondrial Complex II Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).
      Catalogue NumberAAMT002
      Brand Family Novagen®
      References
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Multiple Storage Conditions
      Storage Multiple storage requirements
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      AAMT002 0