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354104 Glutathione Peroxidase, Cellular Assay Kit

Glutathione Peroxidase, Cellular Assay Kit: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
354104
  
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      Descripción

      Description
      Overview

      This product has been discontinued.

      We are offering Glutathione Peroxidase Assay Kit (Cat. No. 353919) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.





      A spectrophotometric assay kit based on the method described by Paglia and Valentine. Glutathione peroxidase (GPx) catalyzes the reduction of an organic peroxide while forming oxidized glutathione (GSSG). GSSG is reduced to GSH by the enzyme glutathione reductase, and NADPH is oxidized to NADP in the catalytic cycle. GPx activity is quantitated by measuring the change in absorbance at 340 nm caused by the oxidation of NADPH.
      Each kit can be used to perform up to 100 assays.
      Catalogue Number354104
      Brand Family Calbiochem®
      Application Data
      Cellular Glutathione Peroxidase at a 288 mU/ml assayed at 23°C.
      Materials Required but Not Delivered• Spectrophotometer, preferably equipped with a temperature-controlled cuvette chamber capable of measuring the absorbance at 340 nm. • Cuvettes with 1-cm path length
      References
      ReferencesThompson, C.D., et al. 1977. Br. J. Nutr. 37, 457. Paglia, D.E., and Valentine, W.N. 1967. J. Lab. Clin. Med. 70, 158.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cause the oxidation of 1.0 μM of NADPH to NADP per minute at 37°C measured at 340 nm.
      Detection methodColorimetric
      Form100 Tests
      FormatCuvette
      Kit containsGlutathione, glutathione reductase, NADPH, t-butylhydroperoxide, buffer, and a user protocol.
      Biological Information
      Assay range5.6-24 mU/ml
      Assay time2 h
      Sample TypeCell lysates, tissue extracts, erythrocyte lysates
      Safety Information
      R PhraseR: 8-20/21/22-34-40-44

      Contact with combustible material may cause fire.
      Harmful by inhalation, in contact with skin and if swallowed.
      Causes burns.
      Limited evidence of a carcinogenic effect.
      Risk of explosion if heated under confinement.
      S PhraseS: 23-36/37/39-16-17

      Do not breathe fumes.
      Wear suitable protective clothing, gloves and eye/face protection.
      Keep away from sources of ignition - No Smoking.
      Keep away from combustible material.
      Storage and Shipping Information
      Ship Code Multiple Storage Conditions
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage Multiple Storage Requirements
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsGlutathione, glutathione reductase, NADPH, t-butylhydroperoxide, buffer, and a user protocol.
      Global Trade Item Number
      Número de referencia GTIN
      354104 0