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L6145-BC Easycoll, Density 1.124 g/ml

L6145-BC
  
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      Descripción

      Replacement Information
      Description
      OverviewThe basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Biochrom AG ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (Cat. No. L 6143/5) can conveniently be prepared with PBS (Cat. No. L 1825).

      Specifications:

      Sterility: Pass
      pH: 7.02-7.62
      Osmolality: 0.281-0.310 Osm/kg H20
      Density: 1.123-1.125 g/ml
      Color: Light Yellow
      Appearance: Clear

      Separating solutions:

      Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation. We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.

      Easycoll

      The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Our ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (Cat. No. L6143/5) can conveniently be prepared with PBS (Cat. No. L1825).

      Production of Easycoll with different densities:

      The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value. But you can also adjust a required density yourself by diluting Easycoll correspondingly.

      Vol.%Eascoll(1.124 g/ml) = D(g/ml-1.005)/1.19 x 103

      The table below shows an example for different densities. All amounts are given for a temperature of +20 °C. Only PBS w/o Ca2+, Mg2+ is suitable, since these ions might influence agglomeration and hence separation of cell clusters. Preparation of solutions with other densities can be made by isoosmolar dilution according to the following formula:

      V[%] = (D'-D) x 102/D"-D%

      where
      D' is required final density
      D" is high starting density
      D is density of isoosmotic dilution solution (g/ml)
      V% is volume percent of the starting solution with high density
      For the dilution we recommend the use of PBS without Ca2+, Mg2+, since both ions can influence the agglomeration of cells and consequently cell separation.
      Catalogue NumberL6145-BC
      Brand Family Calbiochem®
      References
      Product Information
      Quality LevelMQ300
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      L6145-BC 0

      Documentation