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309800 Dihydroethidium - CAS 38483-26-0 - Calbiochem

A cell-permeable, chemically-reduced ethidium derivative.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE

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309800
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Descripción

Tabla espec. clave

CAS #Empirical Formula
38483-26-0C₂₁H₂₁N₃

Precios y disponibilidad

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309800-25MG
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      Description
      OverviewA cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.
      Catalogue Number309800
      Brand Family Calbiochem®
      Synonyms2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE
      References
      ReferencesHaugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
      Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
      Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
      Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
      Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
      Perticarari, S., et al. 1991. Cytometry 12, 687.
      Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
      Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.
      Product Information
      CAS number38483-26-0
      ATP CompetitiveN
      FormPink solid
      Hill FormulaC₂₁H₂₁N₃
      Chemical formulaC₂₁H₂₁N₃
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Biological Information
      Primary TargetUseful fluorogenic probe for the detection of reactive oxygen species (ROS)
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableY
      Safety Information according to GHS
      RTECSSF7935000
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Global Trade Item Number
      Número de referencia GTIN
      309800-25MG 04055977216806