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S8000 CpGenome™ Universal Methylated Mouse DNA Standard Set

The CpGenome Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice & enzymatically methylated at all CpGs by M.SssI methyltransfe

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S8000
5 µg   
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      Descripción

      Description
      Catalogue NumberS8000
      Brand Family Upstate
      Trade Name
      • CpGenome
      • Upstate
      DescriptionCpGenome™ Universal Methylated Mouse DNA Standard Set
      OverviewThe CpGenome™ Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice and enzymatically methylated at all CpGs by M.SssI methyltransferase. It can be used as a positive control for gene methylation studies, and is fully compatible with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847) and CpG WIZ® methylation-specific amplification systems.

      Materials Provided:

      One vial containing 5 µg (20 µl) of CpGenome™ Universal Methylated Mouse DNA Standard at a concentration of 250 ng/µL.

      Validation:

      Methylation-specific PCR (MSP) was performed on the CpGenome™ Universal Methylated Mouse DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® Oct-4 kits (Catalog No. S7840) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for this Standard.

      CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
      Product Information
      PresentationLiquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
      Quality LevelMQ100
      Applications
      ApplicationThe CpGenome Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice & enzymatically methylated at all CpGs by M.SssI methyltransferase.
      Application NotesFor MSP primer design, please use the MethPrime software package. Click here
      Biological Information
      Species Reactivity
      • Mouse
      Modifications
      • Methylation
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended Storage: Stable at -20°C for up to 6 months from date of receipt.
      Packaging Information
      Material Size5 µg
      Global Trade Item Number
      Número de referencia GTIN
      S8000 04053252648397