Millipore Sigma Vibrant Logo

S8001U CpGenome™ Human Non-Methylated DNA Standard Set

It is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
View Products on Sigmaaldrich.com
S8001U
5 µg   
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information
      Description
      Catalogue NumberS8001U
      Brand Family Upstate
      Trade Name
      • CpGenome
      • Upstate
      DescriptionCpGenome™ Human Non-Methylated DNA Standard Set
      OverviewThe CpGenome™ Human Non-Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. It is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome™ Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ® amplification kits.

      Materials Provided:

      One vial containing 5 µg (20 µL) of CpGenome™ Human Non-Methylated DNA Standard at a concentration of 250 ng/µL.

      Validation:

      Methylation-specific PCR (MSP) was performed on the CpGenome™ Human Non-Methylated DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the U primers generated product for this Standard.

      CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
      References
      Product Information
      HS Code2934 99 99
      PresentationLiquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
      Quality LevelMQ100
      Applications
      ApplicationIt is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.
      Application NotesFor MSP primer design, please use the MethPrime software package. Click here
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended Storage: Stable at -20°C for up to 6 months from date of receipt.
      Packaging Information
      Material Size5 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      S8001U 04053252648410

      Documentation

      CpGenome™ Human Non-Methylated DNA Standard Set Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      CpGenome™ Human Non-Methylated DNA Standard Set Certificados de análisis

      CargoNúmero de lote
      CpGenome Human Non-Methylated DNA Standard - Q2023993 Q2023993
      CpGenome™ Human Non-Methylated -2571269 2571269
      CpGenome™ Human Non-Methylated -2802796 2802796
      CpGenome™ Human Non-Methylated DNA Standard 3010026
      CpGenome™ Human Non-Methylated DNA Standard 3126657
      CpGenome™ Human Non-Methylated DNA Standard 3050833
      CpGenome™ Human Non-Methylated DNA Standard 2984242
      CpGenome™ Human Non-Methylated DNA Standard 2930483
      CpGenome™ Human Non-Methylated DNA Standard - 3156112 3156112
      CpGenome™ Human Non-Methylated DNA Standard - 3189686 3189686
      Show more

      Folleto

      Cargo
      Shaping Epigenetics Discovery - DNA Methylation Brochure

      Licencias necesarias e Información técnica

      Cargo
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics