Millipore Sigma Vibrant Logo

218769 Caspase-8, Human, Recombinant, E. coli

Caspase-8, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag® sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: FLICE, MACH, Mch5

View Products on Sigmaaldrich.com
218769
  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information
      Description
      OverviewRecombinant, human caspase-8 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.
      M.W. 11,000 and 18,000 (heterodimer).
      Catalogue Number218769
      Brand Family Calbiochem®
      SynonymsFLICE, MACH, Mch5
      References
      ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Boldin, M.P., et al. 1996. Cell 85, 803.
      Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
      Muzio, M., et al. 1996. Cell 85, 817.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=368057">Cat. No. 368057</a>) per min at 30°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      218769 0

      Documentation

      Caspase-8, Human, Recombinant, E. coli Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Caspase-8, Human, Recombinant, E. coli Certificados de análisis

      CargoNúmero de lote
      218769

      Referencias bibliográficas

      Visión general referencias
      Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Boldin, M.P., et al. 1996. Cell 85, 803.
      Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
      Muzio, M., et al. 1996. Cell 85, 817.

      Folleto

      Cargo
      Caspases and other Apoptosis Related Tools Brochure

      Citas

      Título
    • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
    • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
      		•
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-September-2007 RFH
      SynonymsFLICE, MACH, Mch5
      DescriptionRecombinant, human caspase-8 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
      Purity≥90% by SDS-PAGE
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-pNA (Cat. No. 368057) per min at 30°C, pH 7.4.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°).
      Toxicity Standard Handling
      ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Boldin, M.P., et al. 1996. Cell 85, 803.
      Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464.
      Muzio, M., et al. 1996. Cell 85, 817.
      Citation
    • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
    • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
      		•