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218799 Caspase-6, Human, Recombinant, E. coli

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      Descripción

      Replacement Information
      Description
      OverviewRecombinant, human caspase-6 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
      M.W. 11,000 and 18,000 (heterodimer).
      Catalogue Number218799
      Brand Family Calbiochem®
      SynonymsMch2
      References
      ReferencesLeBlanc, A., et al. 1999. J. Biol. Chem. 274, 23426.
      Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=218762">Cat. No. 218762</a>) per h at 37°C, pH 7.2.
      FormLyophilized
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥6000 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      218799 0

      Documentation

      Caspase-6, Human, Recombinant, E. coli Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Caspase-6, Human, Recombinant, E. coli Certificados de análisis

      CargoNúmero de lote
      218799

      Referencias bibliográficas

      Visión general referencias
      LeBlanc, A., et al. 1999. J. Biol. Chem. 274, 23426.
      Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.

      Folleto

      Cargo
      Caspases and other Apoptosis Related Tools Brochure
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-June-2008 RFH
      SynonymsMch2
      DescriptionRecombinant, human caspase-6 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
      FormLyophilized
      Recommended reaction conditions50 mM HEPES, 50 mM NaCl, 10 mM DTT, 10 mM EDTA. 5% glycerol, and 0.1% CHAPS, pH 7.2.
      Purity≥95% by SDS-PAGE
      Specific activity≥6000 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-pNA (Cat. No. 218762) per h at 37°C, pH 7.2.
      SolubilityReconstitute to 1 unit/µl in PBS, 15% glycerol.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
      Toxicity Standard Handling
      ReferencesLeBlanc, A., et al. 1999. J. Biol. Chem. 274, 23426.
      Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.