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GF101 CD40 Ligand/TRAP Protein, Recombinant human

The CD40 Ligand (CD40L/TRAP protein) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells.

CD40 Ligand/TRAP Protein, Recombinant human: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Key Applications: Cell Culture UniProt Number: P29965 Entrez Gene Number: NM_000074.2
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GF101
10 µg  
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      Descripción

      Tabla espec. clave

      Key ApplicationsSpeciesSourcePurity
      CULTHuman Recombinant humanGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg) of sCD40L/TRAP.
      Description
      Catalogue NumberGF101
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCD40 Ligand/TRAP Protein, Recombinant human
      OverviewCD40 Ligand (CD40L/TRAP) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells. GF101 is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
      Alternate Names
      • CD154
      • TRAP
      • TNF-related activation protein
      • T cell antigen Gp39
      Product Information
      PresentationSterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5.
      Quality LevelMQ100
      Applications
      ApplicationThe CD40 Ligand (CD40L/TRAP protein) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells.
      Key Applications
      • Cell Culture
      Biological Information
      PurityGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg) of sCD40L/TRAP.
      SourceRecombinant human
      Specific ActivityGF101 is fully biologically active as determined by its ability to stimulate the proliferation of HEK-BlueTM CD40 reporter cells.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
      Gene Symbol
      • CD40LG
      • CD154
      • TRAP
      • gp39
      • CD40L
      • IMD3
      • IGM
      • hCD40L
      • TNFSF5
      • HIGM1
      • CD40-L
      • T-BAM
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29965 # Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      SIZE: 261 amino acids; 29274 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & CD40 ligand, soluble form: Secreted.
      TISSUE SPECIFICITY: Specifically expressed on activated CD4+ T- lymphocytes.
      PTM: The soluble form derives from the membrane form by proteolytic processing. & N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. & Not O-glycosylated.
      DISEASE: SwissProt: P29965 # Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
      SIMILARITY: SwissProt: P29965 ## Belongs to the tumor necrosis factor family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized protein is stable for a few weeks for at room temperature but is best stored at -20ºC. Reconstitute in water to 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted sCD40L/TRAP should be stored at -20ºC in undiluted aliquots for up to 6 months. Freeze/thaw cycles will result in significant loss of activity.
      Packaging Information
      Material Size10 µg
      Global Trade Item Number
      Número de referencia GTIN
      GF101 04053252367960