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SCT205 BioTracker™ EP-HMRG Green Protease Live Cell Probe

Live cell imaging probe that reacts with the proteases that digest the peptide sequence of EP (glutamic acid, proline) to fluoresce green by fluorescence microscopy or flow cytometry.

SCT205
5 ea x 30 nmol  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberSCT205
      DescriptionBioTracker™ EP-HMRG Green Protease Live Cell Probe
      OverviewBioTracker™ EP-HMRG is nearly nonfluorescent at pH 7 or higher. It fluoresces by the reaction with proteases that specifically digest the EP (glutamic acid, proline) peptide sequence. A green fluorescent product is membrane permeable and localizes to the lysosome.

      SCT205 exhibits no or very low fluorescence at pH >= 7. It fluoresces by the reaction with proteases that digest the peptide sequces of EP (glutamic acid, proline).

      SPECTRAL PROPERTIES:
      Excitation: 500 nm
      Emission: 524 nm
      For fluorescence microscopy, blue excitation filter cubes for FITC or GFP are suitable.

      REFERENCE

      Onoyama H, Kamiya M, Kuriki Y, Komatsu T, Abe H, Tsuji Y, Yagi K, Yamagata Y, Aikou S, Nishida M, et al. 2016. Rapid and sensitive detection of early esophageal squamous cell carcinoma with fluorescence probe targeting dipeptidylpeptidase IV. Scientific Reports. 6(1):26399.
      Alternate Names
      • EP-HMRG probe
      • EP-HMRG live cell probe
      • protease live cell probe
      • EP protease probe
      Background InformationEarly detection of esophageal squamous cell carcinoma (ESCC) is an important for a favorable prognosis, but is difficult to achieve by conventional endoscopy. Since fluorescence-based visualization is considered a promising approach, development of an activatable fluorescence probe to visualize ESCCs became an urgent goal. Based on the fact that various aminopeptidase activities are elevated in cancer, specimens freshly resected from patients were screened with aminopeptidase-activatable fluorescence probes. The results indicated that dipeptidylpeptidase IV (DPP-IV) is specifically activated in ESCCs and would be an appropriate molecular target for detection of esophageal cancer. DPP-IV-targeted activatable fluorescence probes may prove useful tools for detection of cancers characterized by EP (glutamic acid-proline) sequences such as that found in DPP-IV (dipeptidyl peptidase.)
      References
      Product Information
      Detection methodFlow cytometry Fluorescence
      FormatLyophilized solid
      PresentationLyophilized. Orange solid.
      Quality LevelMQ100
      Applications
      ApplicationLive cell imaging probe that reacts with the proteases that digest the peptide sequence of EP (glutamic acid, proline) to fluoresce green by fluorescence microscopy or flow cytometry.
      Key Applications
      • Protease assays
      • Cell Based Assays
      • Flow Cytometry
      • Fluorescence
      Application Notes
      To use SCT205 EP-HMRG protease live cell probe, warm the vial to room temperature and add 30 μL of DMSO or PBS to one vial to make 1 mM solution.
      Dilute the stock solution with a neutral buffer or culture medium to 1-2 uM and use the diluted solution immediately.

      NOTE: Under acidic conditions, BioTracker™ EP-HMRG Green Protease Live Cell Probe emits fluorescence
      by a different mechanism from protease hydrolysis. It therefore must be diluted with a neutral or weakly basic solution (pH>7).

      Storage of the reconstituted probe solution is not recommended. If it becomes necessary to store temporarily, keep aqueous solution on ice, or freeze DMSO solution and use as quickly as possible.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker™ EP-HMRG Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening.
      NOTE: Storage of reconstituted probe is not recommended. For best results, discard unused probe solution and make fresh for each use.
      Packaging Information
      Material Size5 ea x 30 nmol
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      SCT205 04065270309136