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07-582-I Anti-phospho-IRF-3 (Ser396) Antibody

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07-582-I
200 µL  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-582-I
      Replaces07-582
      DescriptionAnti-phospho-IRF-3 (Ser396) Antibody
      Alternate Names
      • Interferon regulatory factor 3
      • IRF-3
      Background InformationInterferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.
      Product Information
      FormatAffinity Purified
      Control
      • Untreated and sendai virus treated A549 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human IRF-3 phosphorylated at Ser396.
      EpitopeSNS → ANA: Mutation of this residue results in loss of viral-induced phosphorylation of IRF-3.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes IRF-3 phosphorylated at Ser396.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rhesus Macaque and Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IRF3
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~50 kDa observed. Uncharacterized bands may appear at ~40 kDa and ~80 kDa in some lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and sendai virus treated A549 cell lysates.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-IRF-3 (Ser396) in 10 µg of sendai virus treated A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Global Trade Item Number
      Número de referencia GTIN
      07-582-I 04053252599439