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MABN1834 Anti-phospho-Filamin-A (Ser2152) Antibody, clone PS2

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MABN1834
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, ICC, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1834
      DescriptionAnti-phospho-Filamin-A (Ser2152) Antibody, clone PS2
      Alternate Names
      • Filamin-A
      • FLN-A
      • Actin-binding protein 280
      • ABP-280
      • Alpha-filamin
      • Endothelial actin-binding protein
      • Filamin-1
      • Non-muscle filamin
      • phospho-Filamin-A (Ser2152)
      Background InformationFilamin-A (UniProt P21333; also known as ABP-280, Actin binding protein 280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, FLN-A, Non-muscle filamin) is encoded by the FLNA (also known as ABP, CSBS, CVD1, FLN, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD) gene (Gene ID 2316) in human. Filamin-A (FLN-A) is an actin-binding protein essential for cytoskeletal rearrangement and cell locomotion. Serine 2152 of FLN-A is a PKA phosphorylation target. FLN-A S2152 phosphorylation is essential for FLN-A-mediated recruitment of signaling proteins, adhesion molecules, and the vesicle-trafficking complex to the peripheral actin rim, so as to stabilize the cortical structure and epithelial membrane. Mutations in FLNA and ARFGEF2 genes cause periventricular heterotopia (PVH or PH), a congenital malformation of cortical development characterized by a reduced brain size and ectopic neuronal nodules along the ventricular neuroepithelium as a result of impaired initiation of neuronal migration. Suppression of either Arfgef2 or Mekk4 results in increased cellular FLN-A level and Ser2152 phosphorylation. In addition, Arfgef2 or Mekk4 deletion causes PVH in knockout mice.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho-Filamin-A (Ser2152) Antibody, clone PS2 is validated for use in Western Blotting, Immunocytochemistry and Immunofluorescence for the detection of phospho-Filamin-A (Ser2152).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: A representative lot detected enhanced FLN-A Ser2152 phosphorylation in multiple tissue extracts (liver, heart, brain, and skin) from new born (P0) and E16.5 embryos of Arfgef2/Big2 knockout mice when compared with stage-matched tissue samples from wild-type mice (Zhang, J., et al. (2012). J. Neurosci. 32(36):12619-12629).
      Western Blotting Analysis: A representative lot detected cellular FLN-A pSer2152 phosphorylation induction upon serum, forskolin, or EphB2-Fc addition to serum-starved CHP100 human neuroblastoma cells (Zhang, J. et al. (2013). J. Neurosci. 33(40):15735-15746).
      Immunocytochemistry Analysis: A representative lot detected cellular FLN-A pSer2152 phosphorylation induction in neuronal progenitors from frontal cortex of E13.5–E14.5 mouse embryos, CHP100 human neuroblastoma cells, and A7, but not FLN-A-deficient M2, human melanoma cells upon forskolin addition (Zhang, J. et al. (2013). J. Neurosci. 33(40):15735-15746).
      Immunofluorescence Analysis: A representative lot detected FLN-A pSer2152 immunoreactivity extended to the proliferative zone (PZ) surface at the periventricular heterotopia (PVH) congenital malformation site in the forebrain (FB) of E17.5 MEKK-/- mouse embryos in addition to the FLN-A pSer2152 immunoreactivity in the intermediate zone (IZ) and cortical plate (CP) seen in both wild-type and MEKK-knockout embryos by fluorescent immunohistochemistry (Sarkisian, M.R., et al. (2006). Neuron. 52(5):789-801).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the Filamin repeat 20 of human phospho-Filamin-A (Ser2152).
      EpitopeFilamin repeat 20
      ClonePS2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificitySer2152 of human and murine Filamin-A isoform 1 (P21333-1 & Q8BTM8-1) corresponds to Ser2144 of human Filamin-A isoform 2 (P21333-2). Equivalent site is not present in murine spliced isoform 2.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FLNA
      • FLN
      • FLN1
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~280 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse embryonic fibroblast lysate.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected Calyculin A-stimulated Filamin-A Ser2152 phosphorylation in mouse embryonic fibroblasts (MEFs).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABN1834 04055977294347

      Documentation

      Anti-phospho-Filamin-A (Ser2152) Antibody, clone PS2 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-phospho-Filamin-A (Ser2152) Antibody, clone PS2 Certificados de análisis

      CargoNúmero de lote
      Anti-phospho-Filamin-A (Ser2152), clone PS2 - 3845002 3845002
      Anti-phospho-Filamin-A (Ser2152), clone PS2 -Q2610024 Q2610024
      Anti-phospho-Filamin-A (Ser2152), clone PS2 Monoclonal Antibody 3316651

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
      Zhang, J; Neal, J; Lian, G; Hu, J; Lu, J; Sheen, V
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  15735-46  2013

      Mostrar resumen
      24089482 24089482
      Brefeldin A-inhibited guanine exchange factor 2 regulates filamin A phosphorylation and neuronal migration.
      Zhang, J; Neal, J; Lian, G; Shi, B; Ferland, RJ; Sheen, V
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  12619-29  2011

      Mostrar resumen
      22956851 22956851
      MEKK4 signaling regulates filamin expression and neuronal migration.
      Sarkisian, MR; Bartley, CM; Chi, H; Nakamura, F; Hashimoto-Torii, K; Torii, M; Flavell, RA; Rakic, P
      Neuron  52  789-801  2005

      Mostrar resumen
      17145501 17145501

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies