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OP09 Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801)

Descripción

Tabla espec. clave

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
OP09-100UG
Disponibilidad a confirmar
Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      Ampolla de plást. 100 μg
      No pudo obtenerse el precio
      La cantidad mínima tiene que ser múltiplo de
      Maximum Quantity is
      Al finalizar el pedido Más información
      Ahorró ()
       
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      OP09-200UG
      Disponibilidad a confirmar
Disponibilidad a confirmar
      Ingrese cantidad 
      Suspendido
      Cantidades limitadas disponibles
      Debe confirmarse disponibilidad
        El resto: se avisará
          El resto: se avisará
          Se avisará
          Póngase en contacto con el Servicio de Atención al Cliente
          Contact Customer Service

          Ampolla de plást. 200 μg
          No pudo obtenerse el precio
          La cantidad mínima tiene que ser múltiplo de
          Maximum Quantity is
          Al finalizar el pedido Más información
          Ahorró ()
           
          Solicitar precio
          Description
          OverviewRecognizes the ~53 kDa wild-type and mutant p53 protein in A-431 cells and breast carcinoma tissue.
          Catalogue NumberOP09
          Brand Family Calbiochem®
          References
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Negative controlSK-OV-3 cells or normal skin
          Positive controlA431 cells or breast carcinoma tissue
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Application ReferencesOriginal Clone Legros, Y., et al. 1994. Oncogene 9, 2071. Epitope Identification Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.
          Key Applications Frozen Sections
          Gel Shift
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Paraffin Sections
          Application NotesFrozen Sections (5 µg/ml)
          Gel Shift (see comments)
          Immunoblotting (2.5 µg/ml)
          Immunoprecipitation (1 µg/sample)
          Paraffin Sections (5 µg/ml, pepsin, trypsin, or heat pre-treatment required)
          Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53; short incubation times with 35S-Met (≤ 1 h) will help reduce background. For immunoblots of wild-type p53, maximize sensitivity by preconcentrating samples by immunoprecipitation with Cat. No. OP09, then immunoblot using Cat. No. PC35 and chemiluminescent detection. For a gel shift assay, use Cat. No. OP09L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena human p53 fusion protein
          ImmunogenHuman
          Epitopewithin amino acids 46-55 of human p53
          ClonePAb1801
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Global Trade Item Number
          Número de referencia GTIN
          OP09-100UG 04055977225013
          OP09-200UG 04055977225020