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36-012-I-25UL Anti-nitro-α/β-Synuclein (Tyr39) Antibody, clone nSyn14

Anti-nitro-α/β-Synuclein (Tyr39), clone nSyn14, Cat. No. 36-012-I, is a mouse monoclonal antibody that detects α/β-Synuclein nitrated on tyrosine 39 and is tested for use in ELISA, Immunohistochemistry, and Western Blotting.

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36-012-I-25UL
25 µL  
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      Descripción

      Replacement Information
      Description
      Catalogue Number36-012-I-25UL
      DescriptionAnti-nitro-α/β-Synuclein (Tyr39) Antibody, clone nSyn14
      Alternate Names
      • Alpha-synuclein
      • Non-A beta component of AD amyloid
      • Non-A4 component of amyloid precursor
      • NACP
      Background InformationAlpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP; PARK1) gene (Gene ID: 6622) in human. α-synuclein is abundantly expressed in the brain and is found in a classic amyloid fibril form within the intra-neuronal Lewy body deposits of Parkinson's disease brains. In its monomeric form it participates in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. It acts by increasing local calcium release from microdomains, which is essential for the enhancement of ATP-induced exocytosis. In its multimeric form it acts as a molecular chaperone assisting in the folding of synaptic fusion components called SNAREs at presynaptic membrane. It is also shown to regulate dopamine release and transport. It reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Post-translationally it can be phosphorylated, predominantly on serine residues. Phosphorylation of serine 129 is reported to be selective and extensive in synucleinopathy lesions. In addition, increased phosphorylation of Tyr125, nitration of Tyr39, and glycation of α-synuclein have been reported in Parkinson s disease subjects. Nitration of tyrosine residues in α-synuclein is observed in the signature inclusions of Parkinson s disease, dementia with Lewy bodies, the Lewy body variant of Alzheimer s disease, and multiple system atrophy brains. EAAC1 -/- mice display age-dependent loss of dopaminergic neurons in the substantia nigra pars compacta and this neuronal loss is accompanied by increased nitrosylated α-synuclein and microglial activation. Genetic alterations of SNCA gene are reported to result in aberrant polymerization into fibrils, which is associated with several neurodegenerative diseases (synucleinopathies). (Ref.: Miranda, HV., et al. (2017). Sci. Rep. 7; 13713; Berman, AE., et al. (2011). Ann. Neurol. 69(3); 509 520; Giasson, BI., et al. (2000). Science. 290(5493); 985-989).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-nitro-α/β-Synuclein (Tyr39), clone nSyn14, Cat. No. 36-012-I, is a mouse monoclonal antibody that detects α/β-Synuclein nitrated on tyrosine 39 and is tested for use in ELISA, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry
      Application NotesELISA Analysis: A representative lot detected nitro- α/β-Synuclein (Tyr39) in ELISA applications (Giasson, B.I., et al. (2000). Science. 290(5493):985-9).

      Immunohistochemistry Analysis: A representative lot detected nitro- α/β-Synuclein (Tyr39) in Immunohistochemistry applications (Maki, R.A., et al. (2019). Free Radic Biol Med. 141:115-140; Giasson, B.I., et al. (2000). Science. 290(5493):985-9).

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected nitro- α/β-Synuclein in Various a-synuclein protein samples (chemically nitrated or untreated) (Courtesy of Sarah Wright, Nitrome Biosciences, Inc., Brisbane, CA USA).

      ELISA Analysis: Various dilutions of this antibody detected nitro- α/β-Synuclein in various a-synuclein protein samples (chemically nitrated or untreated) (Courtesy of Sarah Wright, Nitrome Biosciences, Inc., Brisbane, CA USA). .

      Western Blotting Analysis: A representative lot detected nitro- α/β-Synuclein (Tyr39) in Western Blotting applications (Miranda, H.V., et al. (2017). Sci Rep. 7(1):13713; Fernandez, E., et al. (2014). Antioxid Redox Signal. 21(15):2143-8; Hung, L.W., et al. (2012). J Exp Med. 209(4):837-54; Giasson, B.I., et al. (2000). Science. 290(5493):985-9).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant human α-synuclein nitrated at tyrosine 39.
      EpitopeN-terminal half
      ClonenSyn14
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone nSyn14 is a mouse monoclonal antibody that detects a/b synuclein nitrated at tyrosine 39.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SCNA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight14.46 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      36-012-I-25UL 04065267780603