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MABC1791-25UG Anti-hMENA11a Antibody, clone A1

Anti-hMENA11a, clone A1, Cat. No. MABC1791, is a mouse monoclonal antibody that detects MENA11a/ENAH and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

MABC1791-25UG
25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABC1791-25UG
      DescriptionAnti-hMENA11a Antibody, clone A1
      Alternate Names
      • Protein enabled homolog
      • hMENA11a
      • Mammalian enabled variant 11a
      • ENAH
      Background InformationProtein enabled homolog (UniProt: A0A097PIC4; also known as hMENA, Mammalian enabled variant 11a, ENAH) is encoded by the ENAH (also known as MENA) gene (Gene ID: 55740) in human. hMENA is a homotetrameric actin regulatory protein of the ENA/VASP family that plays a key role in cell processes that rely on actin cytoskeleton dynamics. Its expression is observed in a variety of tissues. It is overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. Its EVH2 domain mediates tetramerization, F-actin binding, and actin bundle formation. The EVH2 domain is comprised of three regions: Block A (aa 391-411) is a thymosin-like domain that is essential for G-actin binding; block B (aa 442-459) is required for F-actin binding and subcellular location, and block C (aa 554-588) is required for tetramerization. It also contains a KLKR motif (aa 400-403) within block A that is reported to be essential for G-actin binding and actin polymerization. hMENA is absent in normal breast tissue, but it is overexpressed in breast tumors, where its expression correlates with HER-2-positive, ER-negative, and high Ki 67 phenotype. Depletion of hMENA/ hMENA11 is reported to decrease HER3 phosphorylation, inhibits EGF- and NRG-mediated activation of EGFR and HER-2 and counteract growth factor-mediated cell proliferation. Silencing of hMENA/hMENA11a is reported to switch on molecules linked to apoptosis, including cleaved forms of poly (ADP-ribose) polymerase and caspase 9. A hMENA v6 variant of hMENA has also been described that increases cell invasion. In an isogenic model of human breast cancer progression, hMENA11a expression is observed in premalignant cells, whereas hMENA v6 expression is restricted to invasive cancer cells. (Ref.: Melchionna, R., et al. (2020). EMBO Rep. 21; e50078; Trono, P., et al. (2016). Mol. Cell. Oncol. 3(2); e1083648; Di Modugnoa, F., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(47); 19280-19285).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-hMENA11a, clone A1, Cat. No. MABC1791, is a mouse monoclonal antibody that detects MENA11a/ENAH and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesTested Applications

      Immunocytochemistry Analysis: A representative lot detected MENA11a/ENAH in Western Blotting application (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5; Melchionna, R., et al. (2020). EMBO Rep. 21(11):e50078).

      Western Blotting Analysis: A representative lot detected MENA11a/ENAH in Western Blotting applications (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5).

      Immunohistochemistry Applications: A representative lot detected MENA11a/ENAH in Immunohistochemistry applications (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenA linear peptide corresponding to 18 amino acids from the C-terminal region of human MENA protein belonging to 11a exon.
      EpitopeC-terminal
      CloneA1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone A1 is a mouse monoclonal antibody that detects hMENA 11a. It targets an epitope within 18 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ENAH
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~90 kDa observed; 68.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in T47D cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected hMENA11a in T47D cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABC1791-25UG 04065270453631