Millipore Sigma Vibrant Logo

MABF2670-100UG Anti-fHbp Antibody, clone JAR 4

MABF2670-100UG
100 μg  
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
Solicitar precio
Disponibilidad a confirmar
Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Description
      Catalogue NumberMABF2670-100UG
      DescriptionAnti-fHbp Antibody, clone JAR 4
      Alternate Names
      • Meningococcal factor H binding protein
      • Factor H-binding protein
      • Lipoprotein LP2086
      • Lipoprotein GNA1870
      Background InformationFactor H-binding protein (UniProt: Q6QCC2; also known as Lipoprotein LP2086, Lipoprotein GNA1870, and FHbp) is encoded by the gna1870 (also known as fhbp) gene in Neisseria meningitidis. Neisseria meningitidis, an encapsulated Gram-negative bacterium, is an important cause of bacterial sepsis and meningitis. FHbp, a surface exposed lipoprotein, is present in all strains of Neisseria meningitidis but is usually sparsely expressed. It is reported to be the major receptor for human Factor H (FH), a down-regulatory protein of the alternate complement pathway. Recruitment of FH is the major contributory factor to the ability of this bacteria to avoid innate immune response via inhibition of complement-mediated lysis. High affinity between human FH and FHbp is reported to be responsible for sequestering FH in the plasma and down-regulation of the complement system, which in the absence of down-regulation can cause complement-mediated damage to the bacteria. FHbp exists in two major antigenic sub-families known as A and B that do exhibit minimal cross-protective activity. FHbp also has been divided into three variant groups based on its amino acid sequence. Variant group 1 belongs to subfamily B and accounts for about 60% of disease-causing serogroup B isolates. Subfamily A includes variant groups 2 and 3. Antibodies generated against variant 1 are reported to be bactericidal only against bacterial strains expressing FHbp in the variant 1 group but not against strains that express variants 2 and 3 and vice versa. Clone JAR 4 that has a discontinuous epitope does not display bactericidal activity when tested alone. However, it cross-reacts with fHbps in variant group 1 or 2 and is able to elicit human complement-mediated, cooperative bactericidal activity with other non-bactericidal monoclonal antibodies that are specific for fHbp in variant groups 1 and 2. (Ref.: Beernink, PT et al., (2009). Mol. Immunol. 46(8-9); 1647-1653; Welsch, JA., et al. (2004). J. Immunol. 172(9); 5606-5615; Vu, DM et al., (2012). Sci. Rep. 2: 341).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-fHbp, clone JAR 4, Cat. No. MABF2670, is a mouse monoclonal antibody that detects Meningococcal Factor H-binding protein (fHbp) and is tested for use in and Activity Assay, ELISA, Flow Cytometry, and Western Blotting.
      Key Applications
      • Activity Assay
      • ELISA
      • Flow Cytometry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected fHbp in Western Blotting applications (Welsch, J.A., et. al. (2004). J Immunol. 172(9):5606-15).

      Flow Cytometry Analysis: A representative lot detected fHbp in Flow Cytometry applications (Welsch, J.A., et. al. (2004). J Immunol. 172(9):5606-15).

      Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected fHbp in Live bacteria comprising of different strains: Strain H44/76 (ID 1, variant group 1), strain 8047 (ID 77, variant group 2), and strain M1239 (ID 28), variant group 3) (Courtesy of Dan Granoff, M.D. and Serena Giuntini, Ph.D., UCSF Benioff Children s Hospital Oakland California USA).

      ELISA Analysis: A representative lot detected fHbp in ELISA applications (Beernink, P.T., et. al. (2009). Mol Immunol. 46(8-9):1647-53; Vu, D.M., et. al. (2012). Sci Rep. 2:341).

      Activity Assay: A representative lot of this antibody displayed bactericidal activity (Vu, D.M., et. al. (2012). Sci Rep. 2:341).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant Factor H-binding protein (fHbp) from variant group 1 expressed in E. coli.
      EpitopeDiscontinuous from N-terminal
      CloneJAR 4
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityJAR 4 is a mouse monoclonal antibody that detects Factor H-binding protein in Neisseria meningitidis.
      IsotypeIgG2a
      Species Reactivity
      • Bacteria
      Species Reactivity NoteBacteria.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • gna1870
      • fhbp
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight28.99 kDa calculated.
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.



      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Número de referencia GTIN
      MABF2670-100UG 04061841879794