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MABE1925-25UG Anti-acetyl SMC3 (Lys105/106) Antibody, clone 209f/A7

Anti-acetyl SMC3 (Lys105/106), clone 209f/A7, Cat. No. MABE1925, is a mouse monoclonal antibody that detects acetylated Structural maintenance of chromosomes protein 3 (SMC3) and is tested for use in ChIP-seq, Immunofluorescence, and Western Blotting.

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MABE1925-25UG
25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABE1925-25UG
      DescriptionAnti-acetyl SMC3 (Lys105/106) Antibody, clone 209f/A7
      Alternate Names
      • Structural maintenance of chromosomes protein 3
      • SMC protein 3
      • SMC-3
      • Basement membrane-associated chondroitin proteoglycan
      • Bamacan
      • Chondroitin sulfate proteoglycan 6
      • Chromosome-associated polypeptide
      • hCAP
      Background InformationStructural maintenance of chromosomes protein 3 (UniProt: Q9UQE7; also known as SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan, Bamacan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, hCAP) is encoded by the SMC3 (also known as BAM, BMH, CSPG6, SMC3L1) gene (Gene ID: 9126) in human. SMC-3 is a central component of cohesion, a complex required for chromosome cohesion during the cell cycle. Cohesin complexes are composed of the SMC1 and SMC3 heterodimer attached via their SMC hinge domain, RAD21 which link them, and a STAG protein, which interacts with RAD21. Human SMC3 is reported to be acetylated by ESCO1 and ESCO2. Acetylation of SMC3 at lysine 105 and 106 by ESCO1 is important for genomic stability and S phase sister chromatid cohesion. ESCO1 is reported to require precocious dissociation of sisters protein 5 (Pds5), a cohesion regulatory subunit bound to RAD21, to acetylate SMC3 and stabilize chromatin. However, ESCO2 function is not affected by Pds5. Although total SMC3 levels remain stable during cell cycle, acetylated SMC3 declines rapidly during mitosis. The deacetylation of SMC3 is brought about by HDAC8 following anaphase stage. This is important for recycling of cohesin and enables its reloading in interphase for both mitotic and non-mitotic functions. (Ref.: Dasgupta, T., et al. (2016). J. Biol. Chem. 291(24); 12761-12770; Deardorff, MA., et al. (2012). Nature. 489(7415); 313-317; Minamino, M., et al. (2015). Curr. Biol. 25(13); 1694-1706).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-acetyl SMC3 (Lys105/106), clone 209f/A7, Cat. No. MABE1925, is a mouse monoclonal antibody that detects acetylated Structural maintenance of chromosomes protein 3 (SMC3) and is tested for use in ChIP-seq, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • ChIP-seq
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: A representative lot detected acetyl SMC3 (Lys105/106) in Western Blotting applications (Nishiyama, T., et. al. (2010). Cell. 143(5):737-49; Deardorff, M.A., et. al. (2012). Nature. 489(7415):313-7; Minamino, M., et. al. (2015). Curr Biol. 25(13):1694-706; Dasgupta, T., et. al. (2016). J Biol Chem. 291(24):12761-70; Srinivasan, M., et. al. (2018). Cell. 173(6):1508-1519).

      Immunofluorescence Analysis: A representative lot detected acetyl SMC3 (Lys105/106) in Immunofluorescence applications (Dasgupta, T., et. al. (2016). J Biol Chem. 291(24):12761-70).

      ChIP-seq Analysis: A representative lot detected acetyl SMC3 (Lys105/106) in ChIP-seq applications (Srinivasan, M., et. al. (2018). Cell. 173(6):1508-1519; Hill, L., et. al. (2020). Nature. 584(7819):142-147).


      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids surrounding acetylated lysine 105 and 106 from the N-terminal region of human Structural maintenance of chromosomes protein 3 (SMC3).
      EpitopeN-terminal
      Clone209f/A7
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 209f/A7 is a mouse monoclonal antibody that specifically targets Structural maintenance of chromosomes protein 3 (SMC3). It targets acetylated Lysines 105 and 106.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Monkey based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMC3
      • BAM
      • BMH
      • CSPG6
      • SMC3L1
      Modifications
      • Acetylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~160 kDa observed; 141.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa S3 cells treated with Esco1 and Esco2.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected acetyl SMC3 (Lys105/106) in HeLa S3 cells treated with with Esco1 and Esco2, but not in cells treated with siRNA oligos.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABE1925-25UG 04065266020694