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ABC1690-25UG Anti-Wilms Tumor (WT33)

Anti-Wilms Tumor (WT33), Cat. No. ABC1690, is a rabbit polyclonal antibody that detects Wilms tumor protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Wilms Tumor (WT33): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Apoptosis & Cancer Gene Symbol: WT1 UniProt Number: P19544
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ABC1690-25UG
25 μg  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1690-25UG
      DescriptionAnti-Wilms Tumor (WT33)
      Alternate Names
      • WT33
      • WT1
      Background InformationWilms tumor protein (UniProt: P19544; also known as WT33) is encoded by the WT1 gene (Gene ID: 7490) in human. WT33 serves as a transcription factor that plays an important role in cellular development and cell survival. It is expressed in the kidney and a subset of hematopoietic cells. It regulates the expression of numerous target genes, including EPO. WT33 plays an essential role for development of the urogenital system and has a tumor suppressor as well as an oncogenic role in tumor formation. Eight different isoforms of WT33 have been described that are produced by alternative splicing. Isoforms lacking the KTS motif act as transcription factors while isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. The N-terminal region of WT33 contains a proline-rich region (aa 27-83) involved in transcriptional regulation, self-association, and RNA recognition, while its C-terminal region contains four C2H2-type zinc fingers (aa 323-347, 353-377, 383-405, 414-438) that mediate DNA and RNA binding. The zinc finger domain of WT33 can bind to GC-rich sequences, such as the EGR-1 consensus sequence (5 -GCG(T/G)GGGCG-3 ), the WTE motif (5'-GCGTGGGAGT-3'), or (TCC)n motif. Mutations in WT1 gene are reported to cause Wilms tumors characterized by embryonal malignancy of the kidney. Some mutations are reported to cause nephrotic syndrome 4, which causes severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia and edema.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Wilms Tumor (WT33), Cat. No. ABC1690, is a rabbit polyclonal antibody that detects Wilms tumor protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected Wilms Tumor (WT33) in human testis tissue lysate.

      Immunohistochemistry Analysis: A 1: 250-1,000 dilution from a representative lot detected Wilms Wilms Tumor (WT33) in human testis and human kidney tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Wilms Tumor Protein. The immunogen is conserved in isoforms 1,2,3 and 4.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Wilms tumor protein in human. It targets an epitope within 16 amino acids from the N-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • WT1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~59 kDa observed; 49.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Wilms Tumor (WT33) in human kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      ABC1690-25UG 04054839441158