Millipore Sigma Vibrant Logo

MABC609 Anti-Thymidylate Kinase/TMPK Antibody, clone 1A5.1

This Anti-TMPK Antibody, clone 1A5.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Thymidylate Kinase .

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: DTYMK, CDC8, TMPK, TYMK UniProt Number: P23919
View Products on Sigmaaldrich.com
MABC609
100 μg  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information

      Ofertas especiales

      100% Performance Guaranteed

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC609
      DescriptionAnti-Thymidylate Kinase/TMPK Antibody, clone 1A5.1
      Alternate Names
      • Thymidylate kinase
      • Thymidylate Kinase/TMPK
      • dTMP kinase
      Background InformationThymidylate kinase (EC 2.7.4.9; UniProt P23919; also known as dTMP kinase) is encoded by the DTYMK (also known as CDC8, PP3731, TMPK, TYMK) gene (Gene ID 1841) in human. Thymidylate kinase (TMPK) is an ubiquitously expressed enzyme of the dTTP synthesis pathway involved in DNA synthesis. TMPK catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) in the presence of ATP and Mg(2+) to form thymidine 5'-diphosphate (dTDP), which is then further phosphorylated by nucleoside-diphosphate kinase (NDK) to thymidine 5'-triphosphate (dTTP). Aside from its normal physiological role, TMPK activity is essential for the activation of nucleoside analogs-based prodrugs, such as the clinically used anti-HIV prodrugs AZT and d4T, which need to be converted to the triphosphate forms for their pharmacological activities. Inhibition of TMPK activity is reported to augment the potency of anticancer agent doxorubicin against human colon cancer cells regardless of their p53 status. In addition, lung cancers with LKB1-mutations are reported to show deficits in nucleotide metabolism and exhibit hypersensitivity to DTYMK inhibition.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-TMPK Antibody, clone 1A5.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Thymidylate Kinase .
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Thymidylate Kinase/TMPK in 10 µg of MCF-7 cell lysate.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thymidylate Kinase/TMPK in human breast cancer and human ovarian cancer tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Thymidylate Kinase/TMPK.
      Clone1A5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DTYMK
      • CDC8
      • TMPK
      • TYMK
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~24 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Thymidylate Kinase/TMPK in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABC609 04055977175332

      Documentation

      Anti-Thymidylate Kinase/TMPK Antibody, clone 1A5.1 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Thymidylate Kinase/TMPK Antibody, clone 1A5.1 Certificados de análisis

      CargoNúmero de lote
      Anti-Thymidylate Kinase/TMPK, -Q2578154 Q2578154
      Anti-Thymidylate Kinase/TMPK, clone 1A5.1 - 4014611 4014611

      Licencias necesarias e Información técnica

      Cargo
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

      Productos y aplicaciones relacionados

      Related Products

      Número de referencia Descripción  
      ABC458 Anti-Thymidylate Kinase/TMPK Antibody Precios y disponibilidad

      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies