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ST1541 Anti-TPP1 Mouse mAb (3B1)

ST1541
  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~75 kDa TPP1 protein in A431 cells and human salivary gland tissue.
      Catalogue NumberST1541
      Brand Family Calbiochem®
      SynonymsAnti-Triptdyl Peptidase 1
      Application Data
      Detection of human TPP1 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-TPP1 Mouse mAb (3B1) (Cat. No. ST1541) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TPP1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-TPP1 Mouse mAb (3B1) (Cat. No. ST1541) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlA431 cells, Human salivary gland tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 195-305 of human TPP1, expressed as a GST fusion protein
      Clone3B1
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      ST1541 0

      Documentation

      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Triptdyl Peptidase 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human TPP1 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-TPP1 Mouse mAb (3B1) (Cat. No. ST1541) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TPP1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-TPP1 Mouse mAb (3B1) (Cat. No. ST1541) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~75 kDa TPP1 protein.
      BackgroundTPP1 is a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 195-305 of human TPP1, expressed as a GST fusion protein
      Clone3B1
      IsotypeIgG₁
      Specieshuman
      Positive controlA431 cells, Human salivary gland tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C).
      Toxicity Regulatory Review