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ABS2221-100UL Anti-SLC27A4/FATP4

This rabbit polyclonal Anti-SLC27A4/FATP4, Cat. No. ABS2221 is tested for use in Western Blotting, for the detection of Fatty acid transport protein 4 (FATP4).

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ABS2221-100UL
100 µL  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberABS2221-100UL
      DescriptionAnti-SLC27A4/FATP4
      Alternate Names
      • Long-chain fatty acid transport protein 4
      • FATP-4
      • Fatty acid transport protein 4
      • Arachidonate--CoA ligase
      • Long-chain-fatty-acid-CoA ligase
      • Solute carrier family 27 member 4
      • Very long-chain acyl-CoA synthetase 4
      • ACSVL4
      Background InformationLong-chain fatty acid transport protein 4 (UniProt: Q91VE0; also known as FATP-4, Fatty acid transport protein 4, Arachidonate--CoA ligase, Long-chain-fatty-acid-CoA ligase, Solute carrier family 27 member 4, Very long-chain acyl-CoA synthetase 4, ACSVL4) is encoded by the Slc27a4 (also known as AcsvI4, Fatp4) gene (Gene ID: 26569) in murine species. FATP4 is a multi-pass transmembrane protein with an endoplasmic reticulum (ER) localization domain and two transmembrane domains (aa 20-42 and 139-156). It is involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane and has acyl-CoA synthetase activity towards long-chain and very-long-chain fatty acids (VLCFAs). It is abundantly expressed in small intestine, brain kidney, liver, skin, and heart. It is considered as the principal fatty acid transporter in small intestinal enterocytes and is present on the apical side of mature enterocytes. Its expression is also observed in the retinal pigment epithelium and in the retina. It is identified as a negative regulator of the ER-associated retinal pigment epithelium (RPE) 65 isomerase necessary for recycling 11-cis-retinal, the light-sensitive chromophore of both rod and cone opsin visual pigments. FATP4 is reported to indirectly inhibits RPE65 via substrate competition and via production of VLCFA derivatives like lignoceroyl-CoA. Deficiency of FATP4 in RPE is shown to cause a significant increase in the level of 11-cis and 9-cis-retinals that improves dark adaptation and survival function of rods in murine model of Leber congenital amaurosis (LCA). (Ref.: Li, S., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(50); 32114-32123; Newberry, EP., et al. (2003). J. Biol. Chem. 278(51); 51664- 51672; Stahl, A., et al. (1999). Mol. Cell. 4(3); 299-308).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis rabbit polyclonal Anti-SLC27A4/FATP4, Cat. No. ABS2221 is tested for use in Western Blotting, for the detection of Fatty acid transport protein 4 (FATP4).
      Key Applications
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SLC27A4/FATP4 in Mouse liver tissue lysate.

      Western Blotting Analysis: A representative lot detected SLC27A4/FATP4 in Western Blotting applications (Newberry, E.P., et. al. (2003). J Biol Chem. 278(51):51664-72; Li, S., et. al. (2020). Proc Natl Acad Sci USA. 117(50):32114-32123).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenLinear Peptide corresponding to 16 amino acids from the C-terminal region of mouse Fatty acid transport protein 4 (SLC27A4).
      EpitopeC-terminal
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Fatty acid transport protein 4 (SLC27A4). It targets an epitope within 16 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with Monkey, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Slc27a4
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~60 kDa observed; 72.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Hela cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC27A4/FATP4 in Hela cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      ABS2221-100UL 04065267327730