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MAB5552-I-25UG Anti-Pax6 Antibody, clone AD2.38

MAB5552-I-25UG
25 μg  
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      Descripción

      Description
      Catalogue NumberMAB5552-I-25UG
      DescriptionAnti-Pax6 Antibody, clone AD2.38
      Alternate Names
      • Paired box protein Pax-6
      • Aniridia type II protein
      • Oculorhombin
      Background InformationPaired box protein Pax-6 (UniProt: P26367; also known as Aniridia type II protein, Oculorhombin, Pax-6) is encoded by the PAX6 (also known as AN2) gene (Gene ID: 5080) in human. Pax6 is a transcription factor that has important functions in the development of the eye, nose, central nervous system and pancreas. It is shown to be essential for the differentiation of pancreatic islet alpha cells. It has two highly conserved DNA binding domains, a paired domain and a homeodomain that is followed by a proline, serine, and threonine-rich trans-activation domain. Its C-terminal peptide region is shown to modulate DNA binding by the homeodomain. It is expressed in the developing eye and brain and in retina it peaks at fetal days 51-60. Pax6 competes with PAX4 in binding to a common element in the glucagon, insulin, and somatostatin promoters. Pax6 is reported to regulate specification of the ventral neuron subtypes by establishing the correct progenitor domains. Mutations in PAX6 gene are known to cause Aniridia, a disorder characterized by complete absence of the iris or extreme iris hypoplasia. (Ref.: Hanson., IM. (2003). Pediatric Res. 54(6); 791-796; Yasuda, T., et al. (2002). Diabetes. 51(1); 224-230).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Pax6, clone AD2.38, Cat. No. MAB5552-I, is a mouse monoclonal antibody that detects of Paired box protein Pax-6 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Pax6 in human cerebellum tissue sections.

      Immunocytochemistry Analysis: A representative lot detected Pax6 in Immunocytochemistry applications (Guimaraes Melo, R.P., et. al. (2018). Front Cell Neurosci. 12:410).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant human Pax6.
      Epitopeunknown
      CloneAD2.38
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone AD2.38 is a mouse monoclonal antibody that detects Pax-6 protein.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Chicken, Rat, Chicken based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PAX6
      • AN2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 46.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in E13-14 Mouse brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Pax6 in E13-14 Mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Número de referencia GTIN
      MAB5552-I-25UG 04061841878421