Millipore Sigma Vibrant Logo

07-293-I Anti-PICK1

Anti-PICK1, Cat. No. 07-293-I, is a highly specific rabbit polyclonal antibody that targets PRKCA-binding protein and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PICK1: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Gene Symbol: Pick1, Prkcabp UniProt Number: Q62083
View Products on Sigmaaldrich.com
07-293-I
100 µL  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information

      Ofertas especiales

      100% Performance Guaranteed

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-293-I
      DescriptionAnti-PICK1
      Alternate Names
      • PRKCA-binding protein
      • Protein interacting with C kinase 1
      • Protein kinase C-alpha-binding protein
      Background InformationPRKCA-binding protein (UniProt Q62083; also known as Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein) is encoded by the Pick1 (also known as Prkcabp) gene (Gene ID 18693) in murine species. PICK1 is a scaffolding protein expressed in many tissues, where it regulates trafficking of multiple membrane proteins. The PDZ (PSD-95/Dlg/ZO-1/DHR (Dlg homologous region) domain (a.a. 22-105) mediates PICK1 interaction with proteins containing PDZ-binding motifs, such as PKC and acid sensing ion channels (ASICs), while the lipid-binding AH (arfaptin homology)/BAR (Bin/amphiphysin/Rvs) domain (a.a. 144-357) mediates PICK1 homo-dimerization and membrane localization. Pick1 gene-disruption results in downregulated ASICs current and calcium transients during process of ischemia-reperfusion and protects against primary murine cortical neurons against injury caused by OGD-Rep (oxygen and glucose deprivation followed by reperfusion). PICK1 is also shown to act as a negatively regulator against ASIC1 phosphorylation/activation by PKA in rat pulmonary arterial smooth muscle cells, where PICK1 facilitates calcineurin-mediated ASIC1 dephosphorylation.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PICK1, Cat. No. 07-293-I, is a highly specific rabbit polyclonal antibody that targets PRKCA-binding protein and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PICK1 in human cerebellum, kidney, and pancreas tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant mouse PICK1 C-terminal fragment.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody targets the C-terminal region of mouse and rat PICK1, as well as human PICK1 spliced isoform 1, but not isoform 2, reported by UniProt (Q62083, Q9EP80, Q9NRD5).
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pick1
      • Prkcabp
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~55 kDa observed. 46.60 kDa (human/mouse) and 46.61 kDa (rat) calculated. The larger-than-calculated band size is consistent with that reported in the literature (Xia, J., et al. (1999). Neuron. 22(1):179 187).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PICK1 in 10 µg of PC12 rat pheochromocytoma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      07-293-I 04054839057854