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MAB1281A4 Anti-Nuclei Antibody, clone 235-1, Alexa Fluor® 488 conjugate

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MAB1281A4
100 µL  
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      Descripción

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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMAB1281A4
      DescriptionAnti-Nuclei Antibody, clone 235-1, Alexa Fluor® 488 conjugate
      Alternate Names
      • HuNu
      Background InformationCell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat.
      Product Information
      FormatAlexaFluor®488
      Control
      • Human foreskin fibroblasts (SCC058)
      PresentationPurified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
      Quality LevelMQ100
      Applications
      ApplicationThe anti-Nuclei, clone 235-1, Alexa Fluor 488 conjugate will recognize human cell nuclei only & is usefull in xenograph transplantation studies to study stem cell migration.
      Key Applications
      • Immunocytochemistry
      Application NotesEvaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in H9 human embryonic stem cells. Evaluated by Immunocytochemistry in mouse embryonic fibroblasts. Immunocytochemistry Analysis: A 1:50 dilution of this antibody did not detect nuclei in mouse embryonic fibroblasts.
      Biological Information
      ImmunogenHuman nuclei
      CloneClone 235-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in human foreskin fibroblasts (SCC058). Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected nuclei in human foreskin fibroblasts (SCC058).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Número de referencia GTIN
      MAB1281A4 04053252819223