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MABN1618-25UG Anti-Nicotinic Acetylchone Receptor alpha 1 Antibody, clone 7C8.2

Anti-Nicotinic Acetylchone Receptor alpha 1, clone 7C8.2, Cat. No. MABN1618, is a highly specific mouse monoclonal antibody that targets CHRNA1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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Research Category: Neuroscience Gene Symbol: CHRNA1, CHRNA, ACHRA UniProt Number: P02708
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MABN1618-25UG
25 μg  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1618-25UG
      DescriptionAnti-Nicotinic Acetylchone Receptor alpha 1 Antibody, clone 7C8.2
      Alternate Names
      • Acetylcholine receptor subunit alpha
      • AChR nicotinic alpha 1
      Background InformationAcetylcholine receptor subunit alpha (UniProt: P02708; AChR nicotinic alpha 1) is encoded by the CHRNA1 (also known as ACHRA, CHNRA) gene (Gene ID: 1134) in human. Nicotinic Acetylcholine Receptor is a member of the ligan-gated ion channel family. It is a multi-pass membrane protein on the post-synaptic cell membrane. Nicotinic Ach receptor is a pentamer of two alpha chains and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains. Two isoforms of this receptor have been described that are produced by alternative splicing. Isoform 1 is only expressed in skeletal muscle and isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus. It contains an extracellular domain (aa 21-255), four helical domains (aa 256-280; 288-306; 322-341; and 454-472) and a cytoplasmic domain (aa 342-453). After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNA1 gene are linked to Myasthenic syndrome, which is characterized by muscle weakness affecting the axial and limb muscles, and the ocular muscles that lead to ptosis and opthalmoplegia. The alpha subunit is the main focus for antibody binding in myasthenia gravis.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Nicotinic Acetylchone Receptor alpha 1, clone 7C8.2, Cat. No. MABN1618, is a highly specific mouse monoclonal antibody that targets CHRNA1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Nicotinic Acetylchone Receptor alpha 1 in 10 µg of SW480 cell lysate.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Nicotinic Acetylchone Receptor alpha 1 in human colon cancer tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 97 amino acids from the C-terminal half of human nicotinic acetylcholine receptor alpha.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7C8.2 detects nicotinic acetylcholine receptor alpha in human and rat. It targets an epitope within 97 amino acids from the C-terminal half.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • CHRNA1
      • CHRNA
      • ACHRA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~55 kD observed; 54.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in L6 cell lysate.
      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nicotinic Acetylchone Receptor alpha 1 in 10 µg of L6 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABN1618-25UG 04054839494208