Millipore Sigma Vibrant Logo

OP13 Anti-N-Myc Mouse mAb (NCM II 100)

This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc.

Anti-N-Myc Mouse mAb (NCM II 100): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
View Products on Sigmaaldrich.com
OP13
Ver precios y disponibilidad

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
OP13-100UG
Comprobando disponibilidad...
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      		 Ampolla de plást. 100 μg
      Recuperando precio...
      No pudo obtenerse el precio
      La cantidad mínima tiene que ser múltiplo de
      Maximum Quantity is
      Al finalizar el pedido Más información
      Ahorró ()
       
      —
      Solicitar precio
      		 —
      Description
      OverviewRecognizes the N-myc protein and its cleavage product in IMR5 cells.
      Catalogue NumberOP13
      Brand Family Calbiochem®
      References
      ReferencesBrondyk, W.H. 1991. Oncogene 6, 1269.
      Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
      LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
      Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
      Slamon, D.J., et al. 1986. Science 232, 768.
      Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
      Brodeur, G.M., et al. 1984. Science 224, 1121.
      Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
      Kohl, N.E., et al. 1983. Cell 35, 603.
      Schwab, M., et al. 1983. Nature 305, 245.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Positive controlIMR5 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone, Immunofluorescence Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoblotting Brondyk, W.H. 1991. Oncogene 6, 1269. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoprecipitation Brondyk, W.H. 1991. Oncogene 6, 1269.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunofluorescence (1-5 µg/ml, see application references)
      Immunoprecipitation (1-2 µg/sample, see application references)
      Application CommentsIt is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human, N-myc fusion protein, expressed in E. coli
      ImmunogenHuman
      CloneNCM II 100
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      OP13-100UG 04055977224795

      Documentation

      Anti-N-Myc Mouse mAb (NCM II 100) Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-N-Myc Mouse mAb (NCM II 100) Certificados de análisis

      CargoNúmero de lote
      OP13

      Referencias bibliográficas

      Visión general referencias
      Brondyk, W.H. 1991. Oncogene 6, 1269.
      Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
      LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
      Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
      Slamon, D.J., et al. 1986. Science 232, 768.
      Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
      Brodeur, G.M., et al. 1984. Science 224, 1121.
      Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
      Kohl, N.E., et al. 1983. Cell 35, 603.
      Schwab, M., et al. 1983. Nature 305, 245.

      Folleto

      Cargo
      Caspases and other Apoptosis Related Tools Brochure

      Citas

      Título
    • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
      		•
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunofluorescence (1-5 µg/ml, see application references)
      Immunoprecipitation (1-2 µg/sample, see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products.
      BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human, N-myc fusion protein, expressed in E. coli
      CloneNCM II 100
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlIMR5 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsIt is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBrondyk, W.H. 1991. Oncogene 6, 1269.
      Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
      LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
      Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
      Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
      Slamon, D.J., et al. 1986. Science 232, 768.
      Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
      Brodeur, G.M., et al. 1984. Science 224, 1121.
      Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
      Kohl, N.E., et al. 1983. Cell 35, 603.
      Schwab, M., et al. 1983. Nature 305, 245.
      Citation
    • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
      		•
      Application referencesOriginal Clone, Immunofluorescence Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoblotting Brondyk, W.H. 1991. Oncogene 6, 1269. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoprecipitation Brondyk, W.H. 1991. Oncogene 6, 1269.

      Productos y aplicaciones relacionados

      Related Products

      Número de referencia Descripción  
      OP13L Anti-N-Myc Mouse mAb (NCM II 100) Precios y disponibilidad

      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies