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MABE1946-25UL Anti-LOXL2 Antibody, clone GS341

Anti-LOXL2, clone GS341, Cat. No. MABE1946, is a mouse monoclonal antibody that detects LOXL2 and is tested for use in ELISA, Electron Microscopy, Inhibition, and Western Blotting.

MABE1946-25UL
25 µL  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABE1946-25UL
      DescriptionAnti-LOXL2 Antibody, clone GS341
      Alternate Names
      • Lysyl oxidase homolog 2
      • EC: 1.4.3.13
      • Lysyl oxidase-like protein 2
      • Lysyl oxidase-related protein 2
      • Lysyl oxidase-related protein WS9-14
      Background InformationLysyl oxidase homolog 2 (UniProt: Q9Y4K0; also known as EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, LOXL2) is encoded by the LOXL2 gene (Gene ID: 4017) in human. The copper-dependent LOX family contain four LOX homologs (LOXL1, LOXL2, LOXL3, and LOXL4). LOXL2 localizes to both cytoplasmic and nuclear region. It catalyzes the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, it promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 also acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When present in the nucleus, it acts as a transcription corepressor and specifically mediates deamination of trimethylated Lysine 4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Overexpression of LOXL2 has been reported in a number of cancers and its ability to promote epithelial to mesenchymal transition suggest that it might play a role in tumor progression. Higher expression has been correlated with metastasis and reduced survival in patients with aggressive breast cancer. LOXL2 is shown to upregulate in gliomas and drives tumorigenicity by activating autophagy. LOXL2 activity is strongly induced in hypoxia, and it is reported to be a direct transcriptional target of HIF1A. (Ref.: Grossman, M., et al. (2020). Cancer Res; 76(14); 4249-4258; Zhang, Q., et al. (2020). Front. Oncol. 10; 569584; Herranz, N., et al. (2016). FEBS J. 283(23); 4263-4273).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-LOXL2, clone GS341, Cat. No. MABE1946, is a mouse monoclonal antibody that detects LOXL2 and is tested for use in ELISA, Electron Microscopy, Inhibition, and Western Blotting.
      Key Applications
      • Western Blotting
      • Inhibition
      • Enzyme Immunoassay (ELISA)
      • Electron Microscopy
      Application NotesTested applications

      Inhibition: A representative lot of this antibody inhibiter collagen cross-linking in human dermal fibroblasts (HDF).(Grossman, M., et al. (2016). Cancer Res. 76(14); 4249-58).

      Western Blotting Analysis: A representative lot detected LOXL2 in Western Blotting applications (Grossman, M., et al. (2016). Cancer Res. 76(14); 4249-58).

      ELISA Analysis: A representative lot detected LOXL2 in ELISA applications (Grossman, M., et al. (2016). Cancer Res. 76(14); 4249-58).

      Electron Microscopy: A representative lot detected LOXL2 in Electron Microscopy applications (Grossman, M., et al. (2016). Cancer Res. 76(14); 4249-58).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 231 amino acids from the C-terminal half of human Lysyl Oxidase homolog 2 (LOXL2).
      EpitopeC-terminal half
      CloneGS341
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone GS341 is a mouse monoclonal antibody that detects Lysyl Oxidase homolog 2 (LOXL2). It targets an epitope within the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LOXL2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight80 kDa observed; 86.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant Human LOXL2.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant Human LOXL2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABE1946-25UL 04065269802396