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MAB1044 Anti-Interleukin-8 Antibody, clone 8M6

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MAB1044
250 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Gt, ShELISA, FC, NEUT, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1044
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Interleukin-8 Antibody, clone 8M6
      Alternate Names
      • IL-8
      References
      Product Information
      FormatPurified
      PresentationLiquid in PBS containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Interleukin-8 Antibody, clone 8M6 is validated for use in ELISA, FC, NEUT, WB for the detection of Interleukin-8.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Neutralizing
      • Western Blotting
      Application NotesCapture antibody in ELISA. Recommended coating concentration 5 μg/mL. See suggested protocol.

      Flow cytometry: use 1 μg/test

      Neutralizes recombinant ovine IL-8.

      Western blotting

      Optimal working dilutions must be determined by the end user.

      Suggested ELISA Protocol For Ovine IL-8

      1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-8 antibody (Chemicon Catalog Number MAB1044) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.

      2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).

      3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.

      4. Dilute rabbit anti-IL-8 (Chemicon Catalog Number AB1840) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.

      5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.

      6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.

      Notes:

      The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.

      Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.

      Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
      Biological Information
      ImmunogenRecombinant ovine IL-8
      Clone8M6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with ovine IL-8. No reactivity detected to recombinant ovine IL-1beta, IL-6, MCP-1 or TNFalpha.
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Goat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
      Gene Symbol
      • IL8
      • GCP-1
      • emoctakin
      • MDNCF
      • NAP1
      • CXCL8
      • AMCF-I
      • IL-8
      • Emoctakin
      • TSG-1
      • NAP-1
      • LYNAP
      • MONAP
      • SCYB8
      • GCP1
      • LUCT
      • LECT
      • K60
      • 3-10C
      • b-ENAP
      • NAF
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10145 # IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
      SIZE: 99 amino acids; 11098 Da
      SUBUNIT: Homodimer.
      SUBCELLULAR LOCATION: Secreted.
      PTM: Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
      SIMILARITY: SwissProt: P10145 ## Belongs to the intercrine alpha (chemokine CxC) family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MAB1044 04053252616587

      Documentation

      Anti-Interleukin-8 Antibody, clone 8M6 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Interleukin-8 Antibody, clone 8M6 Certificados de análisis

      CargoNúmero de lote
      MOUSE ANTI-OVINE IL-8 MONOCLONAL ANTIBODY - 2428531 2428531
      MOUSE ANTI-OVINE IL-8 - 3178200 3178200
      MOUSE ANTI-OVINE IL-8 - 3183643 3183643
      MOUSE ANTI-OVINE IL-8 - 3245139 3245139
      MOUSE ANTI-OVINE IL-8 - 3386466 3386466
      MOUSE ANTI-OVINE IL-8 - 3445787 3445787
      MOUSE ANTI-OVINE IL-8 - 3666082 3666082
      MOUSE ANTI-OVINE IL-8 - 3766785 3766785
      MOUSE ANTI-OVINE IL-8 - 3809283 3809283
      MOUSE ANTI-OVINE IL-8 - 3959494 3959494

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow.
      Jiemtaweeboon, S; Shirasuna, K; Nitta, A; Kobayashi, A; Schuberth, HJ; Shimizu, T; Miyamoto, A
      Reproductive biology and endocrinology : RB&E  9  79  2010

      Mostrar resumen Artículo Texto completo
      21651784 21651784
      Spermatozoa and seminal plasma induce a greater inflammatory response in the ovine uterus at oestrus than dioestrus.
      Jennifer L Scott,Natkunam Ketheesan,Phillip M Summers
      Reproduction, fertility, and development  21  2009

      Mostrar resumen
      19698286 19698286
      Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.
      Jennifer L Scott,Natkunam Ketheesan,Phillip M Summers
      Reproduction, fertility, and development  19  2007

      Mostrar resumen
      17524304 17524304

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies