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MAB1951 Anti-Integrin β1 Antibody, clone P4G11

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MAB1951
100 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, RbELISA, FC, ICC, IHC, IH(P), IP, FUNC, CULTMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1951
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β1 Antibody, clone P4G11
      Alternate Names
      • CD29
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Tonsil, human skin tissue
      PresentationProtein A Purified immunoglobulin in 0.02M PBS, 0.25M NaCl, pH 7.6, with 0.1% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Integrin β1 Antibody, clone P4G11 is an antibody against Integrin β1 for use in ELISA, FC, IC, IH, IH(P), IP, FUNC, CULT.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Affects Function
      • Cell Culture
      Application NotesImmunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

      Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

      Traditional formalin fixation is not recommended.

      Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

      Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)

      FACS: 2-5μg per 10e6 cells, in the presence of Ca++.

      Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

      ELISA

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman IL-1beta activated endothelial cells
      CloneP4G11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with calcium-dependent epitopes on the integrin β1 subunit. When bound to β1, P4G11, activates β1 dependent binding to extra cellular matrix substrates.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
      Gene Symbol
      • ITGB1
      • GPIIA
      • OTTHUMP00000063731
      • OTTHUMP00000046253
      • OTTHUMP00000063733
      • VLAB
      • OTTHUMP00000063732
      • MDF2
      • FNRB
      • CD29
      • MSK12
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.


      SIZE: 798 amino acids; 88465 Da

      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

      TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).





      SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MAB1951 04053252665646

      Documentation

      Anti-Integrin β1 Antibody, clone P4G11 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Integrin β1 Antibody, clone P4G11 Certificados de análisis

      CargoNúmero de lote
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) MONOCLONAL ANTIBODY - 2392308 2392308
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) - 3352406 3352406
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) - 3600700 3600700
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) - 3745551 3745551
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) - 3868722 3868722
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) - 3892571 3892571
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) -2700975 2700975
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) -2802035 2802035
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) MONOCLONAL ANTIBODY 2880998
      MOUSE ANTI-HUMAN INTEGRIN beta1 (ACTIVATING) MONOCLONAL ANTIBODY 2868047

      Referencias bibliográficas

      Visión general referenciasAplicación EspeciePub Med ID
      Convergence of eicosanoid and integrin biology: 12-lipoxygenase seeks a partner.
      Tang, K; Cai, Y; Joshi, S; Tovar, E; Tucker, SC; Maddipati, KR; Crissman, JD; Repaskey, WT; Honn, KV
      Molecular cancer  14  111  2015

      Mostrar resumen
      26037302 26037302
      Integrins and cAMP mediate netrin-induced growth cone collapse.
      Lemons, ML; Abanto, ML; Dambrouskas, N; Clements, CC; Deloughery, Z; Garozzo, J; Condic, ML
      Brain research  1537  46-58  2013

      Mostrar resumen
      24001590 24001590
      Effect of lumican on the migration of human mesenchymal stem cells and endothelial progenitor cells: involvement of matrix metalloproteinase-14.
      Malinowski, Mariusz, et al.
      PLoS ONE, 7: e50709 (2012)  2011

      Mostrar resumen
      23236386 23236386
      Integrin expression levels correlate with invasion, metastasis and prognosis of oral squamous cell carcinoma.
      Teruhisa Ohara,Shuichi Kawashiri,Akira Tanaka,Natsuyo Noguchi,Hiroko Kitahara,Ayako Okamune,Koroku Kato,Takashi Hase,Hiromitsu Nakaya,Kunio Yoshizawa
      Pathology oncology research : POR  15  2009

      Mostrar resumen
      19130304 19130304
      Role of integrins in the assembly and function of hensin in intercalated cells.
      Vijayakumar, S; Erdjument-Bromage, H; Tempst, P; Al-Awqati, Q
      Journal of the American Society of Nephrology : JASN  19  1079-91  2008

      Mostrar resumen Artículo Texto completo
      Western Blotting, ImmunoprecipitationRabbit18337486 18337486
      Gene expression profile of neuronal progenitor cells derived from hESCs: activation of chromosome 11p15.5 and comparison to human dopaminergic neurons.
      Freed, WJ; Chen, J; Bäckman, CM; Schwartz, CM; Vazin, T; Cai, J; Spivak, CE; Lupica, CR; Rao, MS; Zeng, X
      PloS one  3  e1422  2008

      Mostrar resumen Artículo Texto completo
      18183302 18183302
      FARP2 triggers signals for Sema3A-mediated axonal repulsion.
      Toyofuku, Toshihiko, et al.
      Nat. Neurosci., 8: 1712-9 (2005)  2004

      16286926 16286926
      Regulation of trophoblast beta1-integrin expression by contact with endothelial cells
      Thirkill, TL et al.
      Cell Comm and Signaling, 2(1):4 (2004)  2004

      15189562 15189562
      Snail negatively regulates cell adhesion to extracellular matrix and integrin expression via the MAPK pathway in prostate cancer cells.
      Neal, CL; Mckeithen, D; Odero-Marah, VA
      Cell adhesion & migration  5  249-57  2001

      Mostrar resumen
      21478672 21478672

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies