Millipore Sigma Vibrant Logo

OP133 Anti-IKKα Mouse mAb (14A231)

This Anti-IKKα Mouse mAb (14A231) is validated for use in Immunoblotting for the detection of IKKα.

Anti-IKKα Mouse mAb (14A231): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
View Products on Sigmaaldrich.com
OP133
Ver precios y disponibilidad

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H, Mk, MMMonoclonal Antibody

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
OP133-100UG
Comprobando disponibilidad...
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      		 Ampolla de plást. 100 μg
      Recuperando precio...
      No pudo obtenerse el precio
      La cantidad mínima tiene que ser múltiplo de
      Maximum Quantity is
      Al finalizar el pedido Más información
      Ahorró ()
       
      —
      Solicitar precio
      		 —
      Description
      OverviewRecognizes the ~85 kDa IKKα protein in Daudi cells.
      Catalogue NumberOP133
      Brand Family Calbiochem®
      Application Data
      Detection of human IKKα by immunoblotting. Sample: Whole cell lysate (30 µg) from Daudi cells. Primary antibody: Anti-IKKα Mouse mAb (14A231) (Cat. No. OP133) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesZandi, E., et al. 1998 Science 281, 1360.
      Didonato, J.A., et al. 1997 Nature 388, 548.
      Lee, F.S., et al. 1997 Cell 88, 213.
      Mercurio, F., et al. 1997 Science 278, 860.
      Regnier, C.H., et al. M. 1997 Cell 90, 373.
      Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.2% gelatin, pH 7.4.
      Positive controlDaudi cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-2 µg/ml, chemiluminescence)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag® sequence
      ImmunogenHuman
      Clone14A231
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Monkey
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      OP133-100UG 04055977224801

      Documentation

      Anti-IKKα Mouse mAb (14A231) Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-IKKα Mouse mAb (14A231) Certificados de análisis

      CargoNúmero de lote
      OP133

      Referencias bibliográficas

      Visión general referencias
      Zandi, E., et al. 1998 Science 281, 1360.
      Didonato, J.A., et al. 1997 Nature 388, 548.
      Lee, F.S., et al. 1997 Cell 88, 213.
      Mercurio, F., et al. 1997 Science 278, 860.
      Regnier, C.H., et al. M. 1997 Cell 90, 373.
      Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationImmunoblotting (1-2 µg/ml, chemiluminescence)
      Application Data
      Detection of human IKKα by immunoblotting. Sample: Whole cell lysate (30 µg) from Daudi cells. Primary antibody: Anti-IKKα Mouse mAb (14A231) (Cat. No. OP133) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein.
      BackgroundNF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β and IKK3/γ. In vitro, IKK1/α and IKK2/β can form homo- and heterodimers that can phosphorylate IκBs at the regulatory serine residues directly. IKK1/α and IKK2/β are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK1/α gene in mice results in skin and limb abnormalities and death of newborns.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag® sequence
      Clone14A231
      IsotypeIgG₁
      Specieshuman, monkey, mouse
      Positive controlDaudi cells
      FormLiquid
      FormulationIn PBS, 0.2% gelatin, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesZandi, E., et al. 1998 Science 281, 1360.
      Didonato, J.A., et al. 1997 Nature 388, 548.
      Lee, F.S., et al. 1997 Cell 88, 213.
      Mercurio, F., et al. 1997 Science 278, 860.
      Regnier, C.H., et al. M. 1997 Cell 90, 373.
      Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
      Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.