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MABE243-I Anti-Histone H3. X/Y Antibody, clone 8H6-2111

Anti-Histone H3. X/Y Antibody, clone 8H6-2111, Cat. No. MABE243-I, is a highly specific rat monoclonal antibody that targets Histone H3. X/Y and has been tested in Immunocytochemistry.

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Research Category: Epigenetics & Nuclear Function Gene Symbol: H3.X/Y UniProt Number: N/A
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MABE243-I
100 μg  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE243-I
      ReplacesMABE243
      DescriptionAnti-Histone H3. X/Y Antibody, clone 8H6-2111
      Alternate Names
      • Histone H3 X/Y
      • H3 X/Y
      Background InformationHistones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. It is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. Both H3.X and H3.Y are two novel primate-specific Histone H3 variants that are not found other mammals or lower eukaryotes. H3.X and H3.Y mRNAs are expressed in transformed cell lines, such as U2OS, and in low amounts in human bone, breast and lung tissue. They exhibit amino acid sequence homology with other H3 variants, but differ in several functionally important residues, such as serine10 (S to A) and Serine 28 (S to R). Knockdown of H3.Y variant is shown to affect cell growth and leads to changes in the expression of many genes involved in cell cycle control. It is noteworthy that the number of cells expressing H3.Y is increased under stress stimuli.(Ref.: Wiedemann, SM et al. (2010). J. Cell Biol. 90(5); 777-791).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Histone H3. X/Y Antibody, clone 8H6-2111, Cat. No. MABE243-I, is a highly specific rat monoclonal antibody that targets Histone H3. X/Y and has been tested in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to a sequence of 12 amino acids from the N-terminal region of human Histone H3.X/Y.
      Clone8H6-2111
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 8H6-2111 specifically detects X and Y variants of Histone H3 in primates. It does not recognize HA-H3.1, -H3.2, or -H3.3. This clone targets an epitope of 12 amino acids in the N-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with most primates based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • H3.X/Y
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight17 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in U2OS cells with and without serum starvation.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Histone H3. X/Y in U2OS cells with and without serum starvation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABE243-I 04054839168239