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MABC1786-100UL Anti-Glypican-2 Antibody, clone CT3

Anti-Glypican-2, clone CT3, Cat. No. MABC1786, is a mouse monoclonal antibody that detects Glypican-2 and is tested for use in ELISA, Flow Cytometry, Immunohistochemistry, and Western Blotting.

MABC1786-100UL
100 µL  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABC1786-100UL
      DescriptionAnti-Glypican-2 Antibody, clone CT3
      Alternate Names
      • GPC2
      Background InformationGlypican-2 (UniProt: Q8N158; also known as GPC2) is encoded by the GPC2 gene (Gene ID: 221914) in human. Glypicans belong to a family of heparan sulfate proteoglycans that are attached to the cell membrane via a GPI-anchor. They interact with multiple ligands, including morphogens, growth factors, chemokines, ligands, receptors, and components of the extracellular matrix through their heparan sulfate chains and core protein. GPC2 is synthesized with a signal sequence (aa 1-23), which is subsequently cleaved off to produce the mature GPC2 (aa 24-554). It also contains a propeptide sequence (aa 555-579) that is removed in the mature form. Glypicans are reported to regulate Wnt/β-catenin signaling and affect cell growth and proliferation. Overexpression of GPC2 is reported in several pediatric cancers. Its higher expression is also shown in neuroblastoma compared to the normal peripheral nerve tissue. GPC2 can modulate Wnt signaling and regulate MYCN in neuroblastoma pathogenesis. Genetically silencing of CPC2 is shown to inhibit neuroblastoma cell growth by suppressing N-Myc expression. Clone CT3 specifically binds to GPC2 in human cancer cells and does not react with normal tissues. It recognizes tumor-associated exons 3 and 10 of GPC2. (Ref.: Li, N., et al. (2020). J. Histochem. Cytochem. 68(12); 841-862; Li, N., et al., 2021, Cell Rep. Medicine 2, 100297).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Glypican-2, clone CT3, Cat. No. MABC1786, is a mouse monoclonal antibody that detects Glypican-2 and is tested for use in ELISA, Flow Cytometry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesTested Applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Glypican-2 in IMR-32 cell lysate.

      Immunohistochemistry Applications: A representative lot detected Glypican-2 in Immunohistochemistry applications (Li, N., et al. (2021). Cell Rep Med. 2(6): 100297; Li, N., et al. (2020). J Histochem Cytochem. 68(12): 841-862).

      ELISA: A representative lot detected Glypican-2 in ELISA applications (Li, N., et al. (2021). Cell Rep Med. 2(6): 100297).

      Flow Cytometry Analysis: A representative lot detected Glypican-2 in Flow Cytometry applications (Li, N., et al. (2021). Cell Rep Med. 2(6): 100297; Li, N., et al. (2020). J Histochem Cytochem. 68(12): 841-862).

      Western Blotting Analysis: A representative lot detected Glypican-2 in Western Blotting applications (Li, N., et al. (2021). Cell Rep Med. 2(6):100297).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 50 amino acids from the C-terminal region of human Glypican-2 (GPC2).
      EpitopeC-terminal
      CloneCT3
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone CT3 is a mouse monoclonal antibody that detects Glypican-2 (GPC2) and its glycated forms. It targets an epitope within the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GPC2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight56 kDa observed. 62.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Glypican-2 in SH-SY5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABC1786-100UL 04065270321756