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MABF3125-25UG Anti-Glucuronoxylomannan/GXM Antibody, clone 3E5

Anti-Glucuronoxylomannan/GXM, clone 3E5 , Cat. No. MABF3125, is a mouse monoclonal antibody that detects glucuronoxylomannan/GXM and is tested for use in ELISA, FACS, Immunohistochemistry, and Neutralizing.

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25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABF3125-25UG
      DescriptionAnti-Glucuronoxylomannan/GXM Antibody, clone 3E5
      Alternate Names
      • GXM
      Background InformationGlucuronoxylomannan (GXM), a polysaccharide, is the principal constituent of the Cryptococcus neoformans capsule that modulates the inflammatory response of human monocytes in vitro and exerts many immunoregulatory effects. Its basic structure is a tri-mannose repeat with a glucuronic acid residue in the first mannose. Its structure is shown to be further modified in individual strains by the addition of xylose substitutions on the mannan backbone. Six triads known as M1-M2 are shown to be present in various proportions in GXM from the various serotypes and M2 is reported to be the most common triad in serotype A GXM. Soluble GXM serves as an important virulence factor and contributes to the pathogenesis of infection. High levels of GXM are found in serum of patients with cryptococcosis. Clone 3E5 is a class II monoclonal Abs that is reported to possess hydrolytic activity against a 12 amino acid P1 peptide (SPNQHTPPWMLK) at positions 1, 2, and 10. (Ref.: Bowen, A., et al. (2017). J. Biol. Chem. 292(2); 417-434; Tissi, L., et al. (2004). Infect. Immun. 72(11); 6367-6372; Lendvai, N., and Casadevall, A. (1999). J. Infect. Dis. 180(3); 791-801).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Glucuronoxylomannan/GXM, clone 3E5 , Cat. No. MABF3125, is a mouse monoclonal antibody that detects glucuronoxylomannan/GXM and is tested for use in ELISA, FACS, Immunohistochemistry, and Neutralizing.
      Key Applications
      • ELISA
      • Fluorescence Activated Cell Sorting (FACS)
      • Immunohistochemistry
      • Neutralizing
      Application NotesTested Applicatins

      Neutralizing: A representative lot neutralized Gonoxylomannan/GXM and prevented toxicity in mice. (Lendvai, N., et al. (1999). J Infect Dis.180(3):791-801).

      Immunohistochemistry Applications: A representative lot detected Glucuronoxylomannan/GXM in Immunohistochemistry applications (Feldmesser, M., et al. (1997). J Immunol. 158(2):790-9; Lendvai, N., et al. (1999). J Infect Dis. 180(3):791-801).

      Fluorescence Activated Cell Sorting (FACS): A representative lot was used for sorting J774 and CHO cells labeled with this antibody in Fluorescence Activated Cell Sorting applications (Saylor Hawk, C., et al. (2019). J Immunol. 202(9):2782-2794).

      ELISA Analysis: A representative lot detected Glucuronoxylomannan/GXM in ELISA applications (Janda, A., et al. (2015). J Biol Chem. 290(19):12090-100; Saylor Hawk, C., et al. (2019). J Immunol. 202(9):2782-2794).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGlucuronoxylomannan/GXM from Cryptococcus neoformans conjugated to tetanus toxoid.
      EpitopeUnknown
      Clone3E5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3E5 is a mouse monoclonal antibody that detects Glucuronoxylomannan /GXM in Cryptococcus neoformans infected mouse cells.
      IsotypeIgG1κ
      Species Reactivity
      • Yeast
      Species Reactivity NoteFungus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in Cryptococcus neoformans infected Mouse lung tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected glucuronoxylomannan/GXM in in Cryptococcus neoformans infected Mouse lung tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABF3125-25UG 04065270845177