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371783 Anti-G₁₆α-Subunit, C-Terminal (360-373) Rabbit pAb

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371783
  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the G16α-subunit protein. Supplied with a positive control derived from crude cell membranes from Sf9 cells tranfected with a G16α-expressing construct.
      Catalogue Number371783
      Brand Family Calbiochem®
      References
      ReferencesAmatruda, T.T. 3rd, et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5587.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.4.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (1:50,000-1:100,000)
      Immunoblotting (1:1000)
      Application CommentsThis antibody has fairly broad species cross-reactivity. Variables associated with assay conditions will dictate the proper working dilution.

      25 µl of a G16α Blotting Standard is also supplied, which can be used as a positive control. It is a sample of crude cell membranes from Sf9 cells transformed with a G16α containing virus. The 25 µl sample should be diluted with 250 µl sample buffer and heated for 3 min at 80°C. The recommended loading volume per lane is 25 µl. Use 3% BSA as a blocking agent.
      Biological Information
      Immunogena synthetic peptide [(C)RDSVLARYLDEINL] corresponding to amino acids 360-373 of human, mouse, and rat G₁₆α-subunit
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      371783 0

      Documentation

      Anti-G₁₆α-Subunit, C-Terminal (360-373) Rabbit pAb Certificados de análisis

      CargoNúmero de lote
      371783

      Referencias bibliográficas

      Visión general referencias
      Amatruda, T.T. 3rd, et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5587.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      ApplicationELISA (1:50,000-1:100,000)
      Immunoblotting (1:1000)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the G16α-subunit protein. Supplied with a positive control derived from crude cell membranes from Sf9 cells tranfected with a G16α-expressing construct.
      HostRabbit
      Immunogena synthetic peptide [(C)RDSVLARYLDEINL] corresponding to amino acids 360-373 of human, mouse, and rat G₁₆α-subunit
      IsotypeIgG
      Specieshuman, mouse, rat
      FormLiquid
      FormulationIn PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody has fairly broad species cross-reactivity. Variables associated with assay conditions will dictate the proper working dilution.

      25 µl of a G16α Blotting Standard is also supplied, which can be used as a positive control. It is a sample of crude cell membranes from Sf9 cells transformed with a G16α containing virus. The 25 µl sample should be diluted with 250 µl sample buffer and heated for 3 min at 80°C. The recommended loading volume per lane is 25 µl. Use 3% BSA as a blocking agent.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesAmatruda, T.T. 3rd, et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5587.