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MABC1110-25UG Anti-FGFR4 Antibody, clone BT53

Anti-FGFR4, clone BT53, Cat. No. MABC1110, is a mouse monoclonal antibody that detects FGFR4 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunotoxin, and Inhibition Assay.

MABC1110-25UG
25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABC1110-25UG
      DescriptionAnti-FGFR4 Antibody, clone BT53
      Alternate Names
      • Fibroblast growth factor receptor 4
      • EC: 2.7.10.1
      • CD334
      Background InformationFibroblast growth factor receptor 4 (UniProt: P22455; also known as EC: 2.7.10.1, FGFR-4, CD334) is encoded by the FGRF4 (also known as JTK2, TKF) gene (Gene ID: 2264) in human. FGFR-4 is a highly conserved, single-pass type I membrane protein with tyrosine kinase activity that serves as a cell surface receptor for fibroblast growth factors and regulates cell proliferation differentiation, and migration. FGFR-4 is expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. In the absence of a ligand, it is present as a monomer in an inactive conformation. Upon ligand binding it homodimerizes into an active conformation with autophosphorylation of tyrosine residues. FGFR-4 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 22-369), a transmembrane domain (aa 370-390), and a cytoplasmic domain (aa 391-802). FGFR-4 undergoes N-glycosylation, and when not fully glycosylated, it can undergo proteasomal degradation. FGFR-4 signaling is down-regulated by receptor internalization and degradation. MMP14 is reported to promote internalization and degradation of FGFR-4. Mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling leading to tumor growth. Over-expression of FGFR4 is reported in almost all human prostate cancers and its R388G mutation is associated with tumor progression. It leads to increased receptor stability and sustained receptor activation. (Ref.: Turkington, RC et al. (2014). Cell Death Dis. 5; e1046; Wang, J., et al. (2008). Neoplasia. 10(8); 847-856).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-FGFR4, clone BT53, Cat. No. MABC1110, is a mouse monoclonal antibody that detects FGFR4 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunotoxin, and Inhibition Assay.
      Key Applications
      • Enzyme Immunoassay (ELISA)
      • Flow Cytometry
      • Immunocytochemistry
      • Inhibition
      Application NotesTested Applications

      ELISA Analysis: A representative lot detected Fibroblast growth factor receptor 4 (FGFR4) in ELISA applications.

      Inhibition Assay:: A representative lot inhibited growth, replication, and metastasis in RH30 cells.

      Immunotoxin: A representative lot of this antibody displayed immunotoxin activity as antibody-drug conjugate with Monomethyl auristatin F or Duocamycin DM.

      Flow Cytometry Analysis: A representative lot detected Fibroblast growth factor receptor 4 (FGFR4) in Flow Cytometry applications.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenCells transfected with human FGFR4.
      EpitopeExtracellular domain
      CloneBT53
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone BT53 is a mouse monoclonal antibody that detects FGFR4. It targets an epitope within the extracellular domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FGFR4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight87.95 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Fibroblast growth factor receptor 4 (FGFR4) in HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABC1110-25UG 04065270453518